Abstract
Autophagy is an essential catabolic process that regulates a diverse array of functions by targeting cellular components for degradation by lysosomes. Studies in mammalian cells have shown that the regulation of autophagy is highly complex and optimization of experimental approaches to analyze this process needs to be developed for each model studied. This chapter provides an overview of two of the most commonly used ways to monitor autophagy activity in T cell. It involves description of common techniques, namely Western blot and cell immunostaining, giving specific recommendations for working with T cells and monitoring macroautophagy. We also discuss the analysis required for correct interpretation of the results and quantification of macroautophagy activity.
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This work was supported by NIH grant AG021904.
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Botbol, Y., Macian, F. (2015). Assays for Monitoring Macroautophagy Activity in T cells. In: Shaw, A. (eds) Immunosenescence. Methods in Molecular Biology, vol 1343. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2963-4_12
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DOI: https://doi.org/10.1007/978-1-4939-2963-4_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2962-7
Online ISBN: 978-1-4939-2963-4
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