Abstract
Intracellular bacteria are poorly responsive to antibiotic treatment. Pharmacological studies are thus needed to determine which antibiotics are most potent or effective against intracellular bacteria as well as to explore the reasons for poor bacterial responsiveness. An in vitro pharmacodynamic model is described, consisting of (1) phagocytosis of pre-opsonized bacteria by eukaryotic cells; (2) elimination of non-internalized bacteria with gentamicin; (3) incubation of infected cells with antibiotics; and (4) determination of surviving bacteria by viable cell counting and normalization of the counts based on sample protein content.
*Both authors contributed equally to this work.
J.M. Buyck: Focal Area Infection Biology, Biozentrum, University of Basel, Basel, Switzerland
C. Seral: Department of Microbiology, Hospital ClÚnico Universitario Lozano Blesa, Zaragoza, Spain
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Acknowledgments
Intracellular infection models have been developed thanks to the financial support of the Belgian Fonds National de la Recherche Scientifique, the Interuniversity Attraction Poles initiated by the Belgian Science Policy Office, and the Brussels and Walloon Regions.
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Buyck, J.M. et al. (2016). In Vitro Models for the Study of the Intracellular Activity of Antibiotics. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_13
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DOI: https://doi.org/10.1007/978-1-4939-2854-5_13
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