Abstract
We have developed a method to analyze the functionality of putative TA loci by expressing them in Escherichia coli. Here, we describe the procedure for cloning recombinant TA genes into inducible plasmids and expressing these in E. coli. Following expression, toxicity, resuscitation of growth, and changes in persister cell formation are assayed. This can confirm whether predicted TA loci are active in E. coli and whether expression can affect persister cell formation.
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Butt, A.T., Titball, R.W. (2016). Functional Analysis of the Role of Toxin–Antitoxin (TA) Loci in Bacterial Persistence. In: Michiels, J., Fauvart, M. (eds) Bacterial Persistence. Methods in Molecular Biology, vol 1333. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2854-5_11
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DOI: https://doi.org/10.1007/978-1-4939-2854-5_11
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2853-8
Online ISBN: 978-1-4939-2854-5
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