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Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary

  • Kaitlin M. Laws
  • Daniela Drummond-BarbosaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild-type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/FLP recognition target (FRT)-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.

Keywords

Oogenesis Clonal analysis Genetic mosaics FLP/FRT Germline stem cell Follicle stem cell Follicle cell Drosophila 

Notes

Acknowledgements

We are grateful to members of the Drummond-Barbosa lab for critical comments during the preparation of this manuscript. This work was supported by National Institutes of Health (NIH) grant R01 GM069875 (D.D.B.). K.L. was supported by NIH training grant T32CA009110.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  1. 1.Division of Reproductive Biology, Department of Biochemistry and Molecular BiologyJohns Hopkins Bloomberg School of Public HealthBaltimoreUSA
  2. 2.Department of Environmental Health SciencesJohns Hopkins Bloomberg School of Public HealthBaltimoreUSA

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