Genetic Mosaic Analysis of Stem Cell Lineages in the Drosophila Ovary
- 2k Downloads
Genetic mosaic analyses represent an invaluable approach for the study of stem cell lineages in the Drosophila ovary. The generation of readily identifiable, homozygous mutant cells in the context of wild-type ovarian tissues within intact organisms allows the pinpointing of cellular requirements for gene function, which is particularly important for understanding the physiological control of stem cells and their progeny. Here, we provide a step-by-step guide to the generation and analysis of genetically mosaic ovaries using flippase (FLP)/FLP recognition target (FRT)-mediated recombination in adult Drosophila melanogaster, with a focus on the processes of oogenesis that are controlled by diet-dependent factors.
KeywordsOogenesis Clonal analysis Genetic mosaics FLP/FRT Germline stem cell Follicle stem cell Follicle cell Drosophila
We are grateful to members of the Drummond-Barbosa lab for critical comments during the preparation of this manuscript. This work was supported by National Institutes of Health (NIH) grant R01 GM069875 (D.D.B.). K.L. was supported by NIH training grant T32CA009110.
- 2.Theodosiou NA, Xu T (1998) Use of FLP/FRT system to study Drosophila development. Methods 14:355–365Google Scholar
- 4.Lehmann R, Nusslein-Volhard C (1987) hunchback, a gene required for segmentation of an anterior and posterior region of the Drosophila embryo. Dev Biol 119:402–417Google Scholar
- 11.Ables ET, Drummond-Barbosa D (2010) The steroid hormone ecdysone functions with intrinsic chromatin remodeling factors to control female germline stem cells in Drosophila. Cell Stem Cell 7:581–592Google Scholar
- 19.Spradling AC (1993) Developmental genetics of oogenesis. In: Bate M (ed) The development of Drosophila melanogaster. Cold Spring Harbor Laboratory Press, Plainview, NYGoogle Scholar