Abstract
Drosophila melanogaster oogenesis has emerged as an excellent model system to study multiple aspects of eukaryotic cell biology. Ovarian tissue can easily be isolated and analyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.
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Thompson, L., Randolph, K., Norvell, A. (2015). Basic Techniques in Drosophila Ovary Preparation. In: Bratu, D., McNeil, G. (eds) Drosophila Oogenesis. Methods in Molecular Biology, vol 1328. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2851-4_2
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DOI: https://doi.org/10.1007/978-1-4939-2851-4_2
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2850-7
Online ISBN: 978-1-4939-2851-4
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