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Basic Techniques in Drosophila Ovary Preparation

  • Letitia Thompson
  • Kristen Randolph
  • Amanda NorvellEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

Drosophila melanogaster oogenesis has emerged as an excellent model system to study multiple aspects of eukaryotic cell biology. Ovarian tissue can easily be isolated and analyzed through microscopy or biochemical and molecular biology techniques. Here we describe the isolation of ovarian tissues, techniques to enrich for egg chambers at distinct developmental stages, preparation of protein and nucleic acid extracts, and preparation for microscopic analysis of fixed tissues.

Keywords

Drosophila oogenesis Ovary dissection Staging oocytes Ovary lysate 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Letitia Thompson
    • 1
    • 2
  • Kristen Randolph
    • 1
  • Amanda Norvell
    • 1
    Email author
  1. 1.Department of BiologyThe College of New JerseyEwingUSA
  2. 2.University of Pennsylvania School of Veterinary MedicinePhiladelphiaUSA

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