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Fluorescent In Situ Hybridization of Nuclear Bodies in Drosophila melanogaster Ovaries

  • Zehra F. Nizami
  • Ji-Long Liu
  • Joseph G. GallEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1328)

Abstract

Fluorescent in situ hybridization (FISH) is a technique for determining the cytological localization of RNA or DNA molecules. There are many approaches available for generating in situ hybridization probes and conducting the subsequent hybridization steps. Here, we describe a simple and reliable FISH method to label small RNAs (200–500 nucleotides in length) that are enriched in nuclear bodies in Drosophila melanogaster ovaries, such as Cajal bodies (CBs) and histone locus bodies (HLBs). This technique can also be applied to other Drosophila tissues, and to abundant mRNAs such as histone transcripts.

Keywords

Fluorescence in situ hybridization (FISH) Small nuclear RNAs (snRNAs) Small Cajal body-specific RNAs (scaRNAs) Cajal bodies (CBs) Histone locus bodies (HLBs) Drosophila ovaries 

Notes

Acknowledgements

We thank Svetlana Deryusheva (Carnegie Institution for Science) for useful advice and enhancements to this protocol.

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Zehra F. Nizami
    • 1
  • Ji-Long Liu
    • 2
  • Joseph G. Gall
    • 1
    Email author
  1. 1.Department of EmbryologyCarnegie Institution for ScienceBaltimoreUSA
  2. 2.MRC Functional Genomics Unit, Department of Physiology, Anatomy and GeneticsUniversity of OxfordOxfordUK

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