Skip to main content

Flow Cytometric Analysis of Human Small Intestinal Lymphoid Cells

  • Protocol
Celiac Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1326))

  • 1624 Accesses

Abstract

Flow cytometry is a powerful technique allowing simultaneous analysis of numerous morphological and phenotypic characteristics of cells and cellular constituents. Improvements in cell isolation techniques in recent years have enabled flow cytometric analyses of cells derived from tissue biopsies. Here we describe a method for isolating and analyzing small intestinal lymphoid cells using flow cytometry.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Leon F (2011) Flow cytometry of intestinal intraepithelial lymphocytes in celiac disease. J Immunol Methods 363:177–186. doi:10.1016/j.jim.2010.09.002

    Article  CAS  PubMed  Google Scholar 

  2. Bull DM, Bookman MA (1977) Isolation and functional characterization of human intestinal mucosal lymphoid cells. J Clin Invest 59:966–974. doi:10.1172/JCI108719

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  3. Madrigal L, Lynch S, Feighery C et al (1993) Flow cytometric analysis of surface major histocompatibility complex class II expression on human epithelial cells prepared from small intestinal biopsies. J Immunol Methods 158:207–214

    Article  CAS  PubMed  Google Scholar 

  4. Abuzakouk M, Feighery C, O’Farrelly C (1996) Collagenase and Dispase enzymes disrupt lymphocyte surface molecules. J Immunol Methods 194:211–216

    Article  CAS  PubMed  Google Scholar 

  5. O’Keeffe J, Doherty DG, Kenna T et al (2004) Diverse populations of T cells with NK cell receptors accumulate in the human intestine in health and in colorectal cancer. Eur J Immunol 34:2110–2119. doi:10.1002/eji.200424958

    Article  PubMed  Google Scholar 

  6. Kralovánszky J, Harrington F, Greenwell A, Melnick R (1990) Isolation of viable intestinal epithelial cells and their use for in vitro toxicity studies. In Vivo (Athens, Greece) 4:201–204

    Google Scholar 

  7. Leon F, Roy G (2004) CD7 and CD103 as markers for the clinical enumeration of small-bowel intraepithelial lymphocytes. Scand J Gastroenterol 39:802. doi:10.1080/00365520410003290, author reply 803

    Article  CAS  PubMed  Google Scholar 

  8. Mulder WMC, Koenen H, Muysenberg AJC et al (1994) Reduced expression of distinct T-cell CD molecules by collagenase/DNase treatment. Cancer Immunol Immunother 38:253–258. doi:10.1007/BF01533516

    CAS  PubMed  Google Scholar 

  9. Dunne MR, Elliott L, Hussey S et al (2013) Persistent changes in circulating and intestinal γδ T cell subsets, invariant natural killer T cells and mucosal-associated invariant T cells in children and adults with coeliac disease. PLoS One 8(10), e76008

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  10. Cerf-Bensussan N, Jarry A, Brousse N et al (1987) A monoclonal antibody (HML-1) defining a novel membrane molecule present on human intestinal lymphocytes. Eur J Immunol 17:1279–1285. doi:10.1002/eji.1830170910

    Article  CAS  PubMed  Google Scholar 

  11. Eiras P, Leon F, Camarero C et al (2000) Intestinal intraepithelial lymphocytes contain a CD3−CD7+ subset expressing natural killer markers and a singular pattern of adhesion molecules. Scand J Immunol 52:1–6

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Margaret R. Dunne .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2015 Springer Science+Business Media New York

About this protocol

Cite this protocol

Dunne, M.R. (2015). Flow Cytometric Analysis of Human Small Intestinal Lymphoid Cells. In: Ryan, A. (eds) Celiac Disease. Methods in Molecular Biology, vol 1326. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2839-2_7

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-2839-2_7

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2838-5

  • Online ISBN: 978-1-4939-2839-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics