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Celiac Disease

Methods and Protocols

  • Anthony W. Ryan

Part of the Methods in Molecular Biology book series (MIMB, volume 1326)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Background

    1. Front Matter
      Pages 1-1
    2. Graham D. Turner, Margaret R. Dunne, Anthony W. Ryan
      Pages 3-14
    3. Greg Byrne, Conleth F. Feighery
      Pages 15-22
    4. Josephine M. Ju, Eric V. Marietta, Joseph A. Murray
      Pages 23-33
  3. Laboratory Protocols

    1. Front Matter
      Pages 45-45
    2. Sarah E. J. Cooper, Sharon Wilson, Conleth F. Feighery
      Pages 47-51
    3. Yvonne Kooy-Winkelaar, Frits Koning
      Pages 53-59
    4. Sarah E. J. Cooper, Bashir M. Mohamed, Louise Elliott, Anthony Mitchell Davies, Conleth F. Feighery, Jacinta Kelly et al.
      Pages 67-77
    5. Jillian M. Gahan, Mikaela M. Byrne, Matthew Hill, Emma M. Quinn, Ross T. Murphy, Richard J. L. Anney et al.
      Pages 93-103
    6. Martina Galatola, Renata Auricchio, Luigi Greco
      Pages 105-115
    7. Ben Molloy, Ross McManus
      Pages 117-130
    8. Antoinette S. Perry, Anne-Marie Baird, Steven G. Gray
      Pages 131-158
    9. Ben Molloy, Michael Freeley, Aideen Long, Ross McManus
      Pages 159-173
  4. Bioinformatics

    1. Front Matter
      Pages 175-175
    2. Ciara Coleman, Emma M. Quinn, Ross McManus
      Pages 203-215
    3. Emma M. Quinn, Ross McManus
      Pages 217-232
  5. Back Matter
    Pages 233-235

About this book

Introduction

This volume provides detailed molecular analysis of the associated pathways and cell types involved in Celiac Disease, and embraces new technologies such as next generation sequencing. The book is divided into three parts: Part I gives a background overview with a number of reviews to describe the history and nature of the disease, its diagnosis, the role of animal models, and study designs for investigating genetic susceptibility. Part II describes molecular techniques, including tissue culture, isolation and cloning of relevant cell types, high content analysis of biopsies, and HLA genotyping. The final part of the book begins with an outline of scripting for data management, focusing on tools that are freely available to researchers who wish to explore them. It also describes analysis pipelines for bioinformatics prediction of antigenicity, quality control and analysis of GWAS data, and transcriptome analysis by next generation sequencing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Celiac Disease: Methods and Protocols brings together novel and more traditional methods in molecular biology and immunology, in order to provide a tool-kit for all stages for Celiac Disease research—from the practicalities of identifying, obtaining, stabilizing and maintaining high quality samples, to molecular analysis and bioinformatics.

Keywords

Coeliac Disease HLA genotyping auto-antigen gene expression generation sequencing non-HLA loci reporter assay siRNA knockdown

Editors and affiliations

  • Anthony W. Ryan
    • 1
  1. 1.Trinity Centre for Health SciencesTrinity College Dublin Trinity Centre for Health SciencesDublinIreland

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2839-2
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2838-5
  • Online ISBN 978-1-4939-2839-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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