Abstract
Epigenetic regulation provides the basis for embryonic development, cellular differentiation, and maintenance of cellular function. DNA methylation is involved in various biological phenomena through gene silencing, stabilizing chromosomal structure and suppressing the mobility of retrotransposons. Here, we describe the methods of analyzing DNA methylation by bisulfite-DNA reactions and combined bisulfite restriction analysis (COBRA), as an example study of orexin neurons. These methods are useful for basic and applied neurobiology.
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Hayakawa, K., Hirosawa, M., Shiota, K. (2016). Analysis of DNA Methylation by Bisulfite Reaction in Neural Cells as an Example of Orexin Neurons. In: Karpova, N. (eds) Epigenetic Methods in Neuroscience Research. Neuromethods, vol 105. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2754-8_3
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DOI: https://doi.org/10.1007/978-1-4939-2754-8_3
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2753-1
Online ISBN: 978-1-4939-2754-8
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