Abstract
Sera of tumor patients frequently contain autoantibodies to tumor associated antigens. Here we describe a miniaturized immunoblot platform allowing us to screen sera of patients for the presence of autoantibodies to ten autoantigens in parallel.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Tan EM (1989) Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology. Adv Immunol 44:93–151
Cheever MA, Disis ML, Bernhard H, Gralow JR, Hand SL, Huseby ES et al (1995) Immunity to oncogenic proteins. Immunol Rev 145:33–59
Tan EM (2001) Autoantibodies as reporters identifying aberrant cellular mechanisms in tumorigenesis. J Clin Invest 108:1411–1415
Stockert E, Jager E, Chen YT, Scanlan MJ, Gout I, Karbach J et al (1998) A survey of the humoral immune response of cancer patients to a panel of human tumor antigens. J Exp Med 187:1349–1354
Soussi T (2000) p53 Antibodies in the sera of patients with various types of cancer: a review. Cancer Res 60:1777–1788
Forti S, Scanlan MJ, Invernizzi A, Castiglioni F, Pupa S, Agresti R et al (2002) Identification of breast cancer-restricted antigens by antibody screening of SKBR3 cDNA library using a preselected patient’s serum. Breast Cancer Res Treat 73:245–256
Conrad K, Bachmann M (2006) Tumor-associated autoantibodies. In: Shoenfeld Y, Gershwin ME, Meroni PL (eds) Autoantibodies, 2nd edn. Elsevier, Amsterdam, pp 423–436
Zhang JY, Casiano CA, Peng XX, Koziol JA, Chan EK, Tan EM (2003) Enhancement of antibody detection in cancer using panel of recombinant tumor-associated antigens. Cancer Epidemiol Biomarkers Prev 12:136–143
Lehmann W (2001) Procedure for production of an array for detection of components of a biological sample. Patent DE 101 10 511
Canzler U, Bartsch H, Grossmann K et al (2009) A miniaturized blotting system for simultaneous detecting of different autoantibodies. Methods Mol Biol 536:129–137
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Acknowledgement
This work was supported in part by grants from the Medical Faculty of the TU-Dresden and the BMBF (Germany). We thank Martina Franke for her excellent technical support.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer Science+Business Media New York
About this protocol
Cite this protocol
Canzler, U. et al. (2015). A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7_20
Download citation
DOI: https://doi.org/10.1007/978-1-4939-2694-7_20
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2693-0
Online ISBN: 978-1-4939-2694-7
eBook Packages: Springer Protocols