Western Blotting

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1312)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Edwin Southern
    Pages 1-3
  3. Harry Towbin
    Pages 5-7
  4. W. Neal Burnette
    Pages 9-12
  5. Jaime Renart
    Pages 13-16
  6. Biji T. Kurien, R. Hal Scofield
    Pages 17-30
  7. Biji T. Kurien, Harry Towbin
    Pages 31-40
  8. Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, Daniel J. MacPhee
    Pages 49-60
  9. Graeme Lowe
    Pages 87-92
  10. Rafael F. Pont-Lezica
    Pages 93-104
  11. Sumi Surendran, Annamma Mathai, Vishnampet Venkataraman Radhakrishnan
    Pages 105-108
  12. Biji T. Kurien, R. Hal Scofield
    Pages 109-117
  13. Dalit Talmi-Frank, Charles L. Jaffe, Gad Baneth
    Pages 141-150
  14. Engelbert Buxbaum
    Pages 151-163

About this book


This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.



Antigenic Peptides Centrifuge Blotting Diffusion Blotting Electroblotting Immunoprecipitation Protein Slice Blotting Western Immublotting

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Department of Medicine, University of OklahomaArthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of VeteransOklahoma CityUSA
  2. 2.Department of MedicineArthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical CenterOklahoma CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2694-7
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2693-0
  • Online ISBN 978-1-4939-2694-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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