Abstract
Here we describe the protocols to perform PFGE analysis of chromosomal DNA from the bacterial species Streptococcus pyogenes (group A streptococcus, GAS) after digestion with the restriction enzyme SmaI. Large parts of the procedures are suitable for application to DNA digested with other restriction enzymes as well. We have put an effort to present extensions to solve possible limitations to the discriminatory power of the method in the specific case of S. pyogenes.
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Vitali, L.A., Gherardi, G., Petrelli, D. (2015). Pulsed Field Gel Electrophoresis of Group A Streptococci. In: Jordan, K., Dalmasso, M. (eds) Pulse Field Gel Electrophoresis. Methods in Molecular Biology, vol 1301. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2599-5_12
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DOI: https://doi.org/10.1007/978-1-4939-2599-5_12
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2598-8
Online ISBN: 978-1-4939-2599-5
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