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Cryopreservation of Neonatal Cardiomyocytes

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Cardiomyocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1299))

Abstract

Cardiomyocytes are frequently used for in vitro models for cardiac research. The isolation of cells is time-consuming and, due to the cells limited proliferative abilities, must be performed frequently. To reduce the time requirements and the impact on research animals, we describe a method for cryopreserving neonatal rat cardiomyocytes (NRCMs), and subsequently thawing them for use in assays.

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Acknowledgement

This work was supported by funding from American Heart Association 12BGIA12040477, NC State University Chancellor’s Faculty Excellence Program, and National Natural Science Foundation of China H020381370216.

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Authors and Affiliations

  1. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, 27607, USA

    Adam C. Vandergriff & M. Taylor Hensley

  2. UNC/NCSU Joint Department of Biomedical Engineering, Chapel Hill, NC, USA

    Adam C. Vandergriff & Ke Cheng

  3. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, RB158, 1060 William Moore Drive, Raleigh, NC, 27607, USA

    Ke Cheng

Authors
  1. Adam C. Vandergriff
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  2. M. Taylor Hensley
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  3. Ke Cheng
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Corresponding author

Correspondence to Ke Cheng .

Editor information

Editors and Affiliations

  1. Rochester Institute of Technology, Rochester, New York, USA

    Gary R. Skuse

  2. Rochester Institute of Technology, Rochester, New York, USA

    Maureen C. Ferran

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Vandergriff, A.C., Hensley, M.T., Cheng, K. (2015). Cryopreservation of Neonatal Cardiomyocytes. In: Skuse, G., Ferran, M. (eds) Cardiomyocytes. Methods in Molecular Biology, vol 1299. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2572-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-2572-8_12

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2571-1

  • Online ISBN: 978-1-4939-2572-8

  • eBook Packages: Springer Protocols

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