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  • © 2015

Cardiomyocytes

Methods and Protocols

  • Includes cutting-edge methods and protocols in cardiomyocyte research
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1299)

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Softcover Book USD 119.00
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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
  2. Enrichment of Cardiomyocytes in Primary Cultures of Murine Neonatal Hearts

    • Sreejit Parameswaran, Rajalakshmi Santhakumar, Prasanna Vidyasekar, Rama S. Verma
    Pages 17-25
  3. Methods of Myofibrillogenesis Modeling

    • Nancy K. Drew, Anna Grosberg
    Pages 75-91
  4. Mouse Embryonic Stem Cell-Derived Cardiac Myocytes in a Cell Culture Dish

    • Carley Glass, Reetu Singla, Anshu Arora, Dinender K. Singla
    Pages 145-152
  5. Cryopreservation of Neonatal Cardiomyocytes

    • Adam C. Vandergriff, M. Taylor Hensley, Ke Cheng
    Pages 153-160
  6. Electrotonic Coupled Metabolic Purification of Chick Cardiomyocytes

    • Winston Shim, Haiyang Yu, K. P. Myu Mai Ja, Muhammad Parasuram, Kee Pah Lim, Philip Wong
    Pages 167-175
  7. Gene Transfer into Cardiac Myocytes

    • Sarah E. Lang, Margaret V. Westfall
    Pages 177-190
  8. Back Matter

    Pages 213-216

About this book

This volume has been assembled for scientists interested in basic and applied biomedical research directed toward understanding the development, genetics and function of cardiomyocytes. The methods and protocols contained within it address cell culture techniques, cardiomyocyte differentiation and redifferentiation, experimental induction of cardiomyopathies, introducing genes into cardiomyocytes, genomic approaches to the understanding cardiomyocytes, cryopreservation of neonatal cardiomyocytes and modeling of cardiomyocyte function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.  

Practical and current, Cardiomyocytes: Methods and Protocols explores complimentary areas of cardiomyocyte science that, taken together, can inform individuals with a broad range of interests.

Editors and Affiliations

  • Rochester Institute of Technology, Rochester, USA

    Gary R. Skuse, Maureen C. Ferran

Bibliographic Information

  • Book Title: Cardiomyocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Gary R. Skuse, Maureen C. Ferran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2572-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2571-1Published: 03 April 2015

  • Softcover ISBN: 978-1-4939-4754-6Published: 05 October 2016

  • eBook ISBN: 978-1-4939-2572-8Published: 02 April 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 216

  • Number of Illustrations: 8 b/w illustrations, 28 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access