Abstract
Macroautophagy selectively recycles damaged or unneeded proteins and organelles by degradation via targeting to the autophagosome. The following method seeks to identify candidate Rab GTPases that likely modulate autophagy in PC12 cells during nerve growth factor (NGF) starvation. This microscopy-based assay is a single cell-based quantification of the presence of autophagosomes by fluorescently labeled markers in response to the overexpression of Rabs and mutants in the presence or absence of NGF.
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Acknowledgment
The authors’ research program is supported by the NIH/NIGMS grant R01 GM074692 (to G.L.).
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Marlin, M.C., Li, G. (2015). Differential Effects of Overexpression of Rab5 and Rab22 on Autophagy in PC12 Cells with or without NGF. In: Li, G. (eds) Rab GTPases. Methods in Molecular Biology, vol 1298. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2569-8_25
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DOI: https://doi.org/10.1007/978-1-4939-2569-8_25
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2568-1
Online ISBN: 978-1-4939-2569-8
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