Rab GTPases

Methods and Protocols

  • Guangpu Li

Part of the Methods in Molecular Biology book series (MIMB, volume 1298)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Guangpu Li, M. Caleb Marlin
    Pages 1-15
  3. Yoan Diekmann, José B. Pereira-Leal
    Pages 17-28
  4. Arafath Kaja Najumudeen, Camilo Guzmán, Itziar M. D. Posada, Daniel Abankwa
    Pages 29-45
  5. Andrew J. Lindsay, Stéphanie Miserey-Lenkei, Bruno Goud
    Pages 73-83
  6. Shanshan Feng, Bin Wu, Johan Peränen, Wei Guo
    Pages 99-106
  7. Juan Wang, Serena Cervantes, Saralin Davis, Susan Ferro-Novick
    Pages 117-125
  8. Takao Yasuda, Paulina S. Mrozowska, Mitsunori Fukuda
    Pages 127-139
  9. Matias A. Bustos, Ornella Lucchesi, Maria C. Ruete, Luis S. Mayorga, Claudia N. Tomes
    Pages 141-160
  10. Andrew J. Lindsay, Mary W. McCaffrey
    Pages 161-171
  11. Shreya Mitra, Jennifer Molina, Gordon B. Mills, Jennifer B. Dennison
    Pages 195-205
  12. Hotaka Kobayashi, Kan Etoh, Soujiro Marubashi, Norihiko Ohbayashi, Mitsunori Fukuda
    Pages 207-216
  13. Patrick D. Allaire, Peter S. McPherson, Brigitte Ritter
    Pages 217-231

About this book


This volume covers the latest technological advances in the characterization of the biosynthesis and functions of Rab GTPases and their regulation by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). The book consists of 28 chapters, and starts with an overview of the Rab GTPase family. The next few chapters describe systematic approaches to the identification and classification of Rabs and Rab GAPs, as well as the detection of Rab isoprenylation and membrane distribution. The last few chapters examine the biochemical and functional properties of individual Rabs in the order of exocytic, recycling, and endocytic Rabs. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Instructive and practical, Rab GTPases: Methods and Protocols approaches each topic with great detail and is a valuable resource for researchers and students interested in the field of Rab GTPases.


Endocytic Pathways Eukaryotes Exocytic Pathways GTPase-Activating Proteins Guanine Nucleotide Exchange Factors Membrane Trafficking Rab GTPase Cycle Rab Genes Vesicular Transport

Editors and affiliations

  • Guangpu Li
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Peggy and Charles Stephenson Cancer CenterUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2569-8
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2568-1
  • Online ISBN 978-1-4939-2569-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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