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Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors

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NF-kappa B

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1280))

Abstract

The transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is crucial for immune responses and skeletal development. Work in recent years has shown that various members of the NF-κB family are viable targets to regulate activity and survival of bone cells and hence bone metabolism. In this regard, deletion of upstream kinases or distal NF-κB subunits resulted with bone deformities. Thus, it has become increasingly apparent that detailed investigation of NF-κB in bone cells may provide opportunities to design new therapeutic modalities. In this chapter we present modified methodology describing efficient approaches to regulate the NF-κB pathway in vitro and in vivo to assess its function in bone cells and tissues.

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Acknowledgments

Work in the authors’ laboratory was supported by R01 AR049192, AR054329 (NIH/NIAMS), and 85100 from the Shriners Hospital for Children.

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Authors and Affiliations

  1. Department of Orthopedic Surgery-Research, Washington University School of Medicine, 660 S. Euclid Ave, Campus Box 8233, Saint Louis, MO, 63110, USA

    Gaurav Swarnkar & Yousef Abu-Amer Ph.D.

  2. Department of Cell Biology & Physiology, Washington University School of Medicine, 660 S. Euclid Ave, Campus Box 8233, Saint Louis, MO, 63110, USA

    Gaurav Swarnkar

Authors
  1. Gaurav Swarnkar
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  2. Yousef Abu-Amer Ph.D.
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Corresponding author

Correspondence to Yousef Abu-Amer Ph.D. .

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Editors and Affiliations

  1. Department of Animal Biology, University of Pennsylvania School of Veterinary Medicine, Philadelphia, Pennsylvania, USA

    Michael J. May

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Swarnkar, G., Abu-Amer, Y. (2015). Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors. In: May, M. (eds) NF-kappa B. Methods in Molecular Biology, vol 1280. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2422-6_31

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  • DOI: https://doi.org/10.1007/978-1-4939-2422-6_31

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2421-9

  • Online ISBN: 978-1-4939-2422-6

  • eBook Packages: Springer Protocols

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