NF-kappa B

Methods and Protocols

  • Michael J. May

Part of the Methods in Molecular Biology book series (MIMB, volume 1280)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Standard Approaches to Detect NF-κB Pathway Activation

    1. Front Matter
      Pages 1-1
    2. Sitharam Ramaswami, Matthew S. Hayden
      Pages 3-13
    3. Petro Starokadomskyy, Ezra Burstein
      Pages 15-24
  3. Detection and Analysis of NF-κB Signaling

    1. Front Matter
      Pages 45-45
    2. Amy Colleran, Patricia E. Collins, Ruaidhrí J. Carmody
      Pages 47-59
    3. Anca B. Mihalas, Mollie K. Meffert
      Pages 61-74
    4. Changying Jiang, Xin Lin
      Pages 75-102
    5. Zhaoxia Qu, Gutian Xiao
      Pages 121-154
    6. Carolyn M. Gray, Michael J. May
      Pages 181-195
  4. Methods to Study the Control of NF-κB Signaling

    1. Front Matter
      Pages 217-217
    2. Daniel Nagel, Daniel Krappmann
      Pages 239-246
    3. Anna D. Reichardt, Jose Pindado, Shivam A. Zaver, Genhong Cheng
      Pages 247-268
    4. Eugene Varfolomeev, Tatiana Goncharov, Domagoj Vucic
      Pages 269-282

About this book


Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function. NF-kappa B: Methods and Protocols guides readers through standard approaches to detect NF-κB pathway activation, detection and analysis of NF-κB signaling, and methods to study the control of NF-κB signaling. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, NF-Kappa B: Methods and Protocols provides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.


DELFIA Electrophoretic Mobility Shift Epidermal Growth Factor (EGF) IKK activity NF-κB pathway activation NF-κB pathway signaling proteins T cell receptor (TCR) chromatin immunoprecipitation (ChIP) microscale thermophoresis non-canonical NF-κB pathway

Editors and affiliations

  • Michael J. May
    • 1
  1. 1.Department of Animal BiologyUniversity of Pennsylvania School of Veterinary MedicinePhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2421-9
  • Online ISBN 978-1-4939-2422-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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