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How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag

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Site-Specific Protein Labeling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1266))

Abstract

The biarsenical-tetracysteine tagging system was the first of (and inspiration for) the now numerous methods for site-specifically labeling proteins in living cells with small molecules such as fluorophores. This historical recollection describes its conception and the trial-and-error chemical development required to become a versatile technique.

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Author information

Authors and Affiliations

  1. Independent Researcher, 4470 Arch Street, San Diego, CA, USA

    B. Albert Griffin

  2. Department of Pharmacology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0647, USA

    Stephen R. Adams & Roger Y. Tsien

  3. Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

    Roger Y. Tsien

  4. Howard Hughes Medical Institute, University of California San Diego, La Jolla, CA, USA

    Roger Y. Tsien

Authors
  1. B. Albert Griffin
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  2. Stephen R. Adams
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  3. Roger Y. Tsien
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Corresponding author

Correspondence to Roger Y. Tsien .

Editor information

Editors and Affiliations

  1. Department of Chemistry, École Normale Supérieure, Paris, France

    Arnaud Gautier

  2. Sensitive Farbstoffe GbR, Munich, Germany

    Marlon J. Hinner

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Griffin, B.A., Adams, S.R., Tsien, R.Y. (2015). How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag. In: Gautier, A., Hinner, M. (eds) Site-Specific Protein Labeling. Methods in Molecular Biology, vol 1266. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2272-7_1

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  • DOI: https://doi.org/10.1007/978-1-4939-2272-7_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2271-0

  • Online ISBN: 978-1-4939-2272-7

  • eBook Packages: Springer Protocols

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