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Site-Specific Protein Labeling

Methods and Protocols

  • Book
  • © 2015

Overview

Editors:
  1. Arnaud Gautier

    1. Department of Chemistry, École Normale Supérieure, Paris, France

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    You can also search for this editor in PubMed   Google Scholar

  2. Marlon J. Hinner

    1. Sensitive Farbstoffe GbR, Munich, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts
  • Includes supplementary material: sn.pub/extras

Part of the book series: Methods in Molecular Biology (MIMB, volume 1266)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag

    • B. Albert Griffin, Stephen R. Adams, Roger Y. Tsien
    Pages 1-6

  3. Site-Specific Protein Labeling in the Pharmaceutical Industry: Experiences from Novartis Drug Discovery

    • Lukas Leder
    Pages 7-27

  4. Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

    • Nicole J. Yang, Marlon J. Hinner
    Pages 29-53

  5. Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes

    • Ivan R. Corrêa Jr.
    Pages 55-79

  6. 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues

    • Lina Cui, Jianghong Rao
    Pages 81-92

  7. Fluorescent Labeling for Patch-Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels

    • Thomas K. Berger, Ehud Y. Isacoff
    Pages 93-106

  8. Fluorescent Labeling of SNAP-Tagged Proteins in Cells

    • Gražvydas Lukinavičius, Luc Reymond, Kai Johnsson
    Pages 107-118

  9. HaloTag Technology for Specific and Covalent Labeling of Fusion Proteins

    • Hélène A. Benink, Marjeta Urh
    Pages 119-128

  10. Ligation of Synthetic Peptides to Proteins Using Semisynthetic Protein trans-Splicing

    • Julian C. J. Matern, Anne-Lena Bachmann, Ilka V. Thiel, Gerrit Volkmann, Alexandra Wasmuth, Jens Binschik et al.
    Pages 129-143

  11. Chemical-Tag Labeling of Proteins Using Fully Recombinant Split Inteins

    • Anne-Lena Bachmann, Julian C. J. Matern, Vivien Schütz, Henning D. Mootz
    Pages 145-159

  12. Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase-Catalyzed Site-Specific Protein Labeling

    • Bo Zhao, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang et al.
    Pages 161-170

  13. Site-Specific Biotinylation of Purified Proteins Using BirA

    • Michael Fairhead, Mark Howarth
    Pages 171-184

  14. Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist

    • Maximilian Wei-Lin Popp
    Pages 185-198

  15. BONCAT: Metabolic Labeling, Click Chemistry, and Affinity Purification of Newly Synthesized Proteomes

    • Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, Daniela C. Dieterich
    Pages 199-215

  16. Genetic Encoding of Unnatural Amino Acids for Labeling Proteins

    • Kathrin Lang, Lloyd Davis, Jason W. Chin
    Pages 217-228

  17. Labeling Proteins by Affinity-Guided DMAP Chemistry

    • Tomonori Tamura, Itaru Hamachi
    Pages 229-242

  18. Ligand-Directed Tosyl Chemistry for Selective Native Protein Labeling In Vitro, In Cells, and In Vivo

    • Shinya Tsukiji, Itaru Hamachi
    Pages 243-263

  19. Back Matter

    Pages 265-267
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Keywords

About this book

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.

Editors and Affiliations

  • Department of Chemistry, École Normale Supérieure, Paris, France

    Arnaud Gautier

  • Sensitive Farbstoffe GbR, Munich, Germany

    Marlon J. Hinner

Bibliographic Information

  • Book Title: Site-Specific Protein Labeling

  • Book Subtitle: Methods and Protocols

  • Editors: Arnaud Gautier, Marlon J. Hinner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-2272-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2015

  • Hardcover ISBN: 978-1-4939-2271-0Published: 06 January 2015

  • Softcover ISBN: 978-1-4939-4417-0Published: 24 September 2016

  • eBook ISBN: 978-1-4939-2272-7Published: 05 January 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 267

  • Number of Illustrations: 38 b/w illustrations, 34 illustrations in colour

  • Topics: Protein Science, Protein Structure

  • Industry Sectors: Biotechnology, Pharma

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