Abstract
The development of simple, inexpensive paper-based sensors for medical diagnostics and other applications is now an important emerging area in the field of biosensors; however, the electronic instrument or reader used to interrogate such sensors adds significantly to the cost of the analysis. In this chapter we describe the design and construction of novel, low-cost disposable electrochemiluminescent (ECL) sensors based on screen printed carbon electrodes and paper-based microfluidics. Moreover, a method to interrogate these sensors using only a mobile phone is articulated. This is realized by exploiting the audio output of the device to achieve electrochemical control, while using the camera to detect the resulting light emitted during the ECL reaction. The combination of cell phone technology with low-cost paper microfluidic sensors dramatically reduces the cost of sensing and has the potential to enhance health-care outcomes by exploiting the functionality, connectivity, and close to worldwide penetration of mobile phone technology.
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References
http://data.worldbank.org/. Accessed 28 May 2013
Martinez AW, Phillips ST, Carrilho E, Thomas SW III, Sindi H, Whitesides GM (2008) Simple telemedicine for developing regions: camera phones and paper-based microfluidic devices for real time, off-site diagnosis. Anal Chem 80:3699–3707
Dungchai W, Chailapakul O, Henry CS (2009) Electrochemical detection for paper-based microfluidics. Anal Chem 81:5821–5826
Barbante GJ, Hogan CF, Wilson DJD, Lewcenko NA, Pfeffer FM, Barnett NW, Francis PF (2011) Simultaneous control of spectroscopic and electrochemical properties in functionalised electrochemiluminescent tris (2,2′-bipyridine) ruthenium (ii) complexes. Analyst 136:1329–1338
Piper DJE, Barbante GJ, Brack N, Pigram PJ, Hogan CF (2011) Highly stable ECL active films formed by the electrografting of a diazotized ruthenium complex generated in situ from the amine. Langmuir 27:474–480
Doeven EH, Zammit EM, Barbante GJ, Francis PF, Barnett NW, Hogan CF (2013) A potential-controlled switch on/off mechanism for selective excitation in mixed electrochemiluminescent systems. Chem Sci 4:977–982
Barbante GJ, Hogan CF, Mechler A, Hughes AB (2010) Electrochemiluminescence of surface bound microparticles of ruthenium complexes. J Mater Chem 20:891–899
Delaney JL, Hogan CF, Tian J, Shen W (2011) Electrogenerated chemiluminescence detection in paper-based microfluidic sensors. Anal Chem 83:1300–1306
Delaney JL, Doeven EH, Harsant AJ, Hogan CF (2013) Use of a mobile phone for potentiostatic control with low cost paper-based microfluidic sensors. Anal Chim Acta 790:56–60
Li X, Tian J, Garnier G, Shen W (2010) Fabrication of paper-based microfluidic sensors by printing. Colloids Surf B Biointerfaces 76:564–570
Fenton EM, Mascarenas MR, Lopez GP, Sibbett SS (2009) Multiplex lateral-flow test strips fabricated by two-dimensional shaping. ACS Appl Mater Interfaces 1:124–129
http://rsbweb.nih.gov/ij/index.html. Accessed 13 Feb 2014
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Delaney, J.L., Hogan, C.F. (2015). Mobile Phone Based Electrochemiluminescence Detection in Paper-Based Microfluidic Sensors. In: Rasooly, A., Herold, K. (eds) Mobile Health Technologies. Methods in Molecular Biology, vol 1256. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2172-0_19
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DOI: https://doi.org/10.1007/978-1-4939-2172-0_19
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