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A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging

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Apoptosis and Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1219))

Abstract

In vitro cell proliferation and apoptosis assays are widely used to study cancer cell biology. Commonly used methodologies are however performed at a single, user-defined endpoint. We describe a kinetic multiplex assay incorporating the CellPlayerTM NucLight Red reagent to measure proliferation and the CellPlayerTM Caspase-3/7 reagent to measure apoptosis using the two-color, live-content imaging platform, IncuCyteTM ZOOM. High-definition phase-contrast images provide an additional qualitative validation of cell death based on morphological characteristics. The kinetic data generated using this strategy can be used to derive informed pharmacology measurements to screen potential cancer therapeutics.

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References

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Author information

Authors and Affiliations

  1. Essen Bioscience, 300 West Morgan Road, Ann Arbor, MI, 48108, USA

    Katherine Artymovich & Daniel M. Appledorn

Authors
  1. Katherine Artymovich
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  2. Daniel M. Appledorn
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Corresponding author

Correspondence to Daniel M. Appledorn .

Editor information

Editors and Affiliations

  1. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, Connecticut, USA

    Gil Mor

  2. Department of Obstetrics, Gynecology, and Reproductive Sciences,, Yale University, New Haven, Connecticut, USA

    Ayesha B. Alvero

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© 2015 Springer Science+Business Media New York

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Artymovich, K., Appledorn, D.M. (2015). A Multiplexed Method for Kinetic Measurements of Apoptosis and Proliferation Using Live-Content Imaging. In: Mor, G., Alvero, A. (eds) Apoptosis and Cancer. Methods in Molecular Biology, vol 1219. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1661-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-1661-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1660-3

  • Online ISBN: 978-1-4939-1661-0

  • eBook Packages: Springer Protocols

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