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Evaluation of Synovial Mast Cell Functions in Autoimmune Arthritis

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Mast Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1220))

Abstract

Mast cells are innate immune effector cells that reside in the healthy synovial sublining and expand in number with inflammation. These cells can play an important role in initiation of arthritis, but much about their biology and importance remains obscure. This chapter reviews the use of animal models for the study of mast cells in arthritis, with a particular focus on the K/BxN serum transfer model. We discuss tissue preparation and histological analysis for the assessment of joint inflammation, injury, and the presence and phenotype of synovial mast cells, as well as the use of bone marrow-derived mast cell (BMMC) engraftment into W/Wv mice as a tool to isolate the role of mast cells in joint inflammation and injury.

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Acknowledgements

This work was funded in part through the support of the Cogan Family Foundation (to P.A.N.). We are grateful to Dr. Altan Ercan for the K/BxN IgG ELISA protocol, to Ms. Theresa Bowman for histotechnical guidance, and to Dr. Nancy Kedersha for the immunofluorescence mounting medium protocols.

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Authors and Affiliations

  1. Division of Rheumatology, Immunology and Allergy, Brigham and Women’s Hospital, Smith 516B, One Jimmy Fund Way, Boston, MA, 02115, USA

    Peter A. Nigrovic M.D.

  2. Division of Immunology, Boston Children’s Hospital, Smith 516B, One Jimmy Fund Way, Boston, MA, 02115, USA

    Peter A. Nigrovic M.D.

  3. Division of Rheumatology, SMG-SNU Borame Medical Center, 20 Boramae-ro-5-gil, Dongjak-gu, Seoul, 156-707, Korea

    Kichul Shin M.D., Ph.D.

Authors
  1. Peter A. Nigrovic M.D.
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  2. Kichul Shin M.D., Ph.D.
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Corresponding author

Correspondence to Peter A. Nigrovic M.D. .

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Editors and Affiliations

  1. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Michael R. Hughes

  2. Department of Medical Genetics, The Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada

    Kelly M. McNagny

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Nigrovic, P.A., Shin, K. (2015). Evaluation of Synovial Mast Cell Functions in Autoimmune Arthritis. In: Hughes, M., McNagny, K. (eds) Mast Cells. Methods in Molecular Biology, vol 1220. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1568-2_26

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  • DOI: https://doi.org/10.1007/978-1-4939-1568-2_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1567-5

  • Online ISBN: 978-1-4939-1568-2

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