Abstract
The identification of suitable protein structures that can serve as scaffolds for the introduction of catalytic residues is crucial for the design of new enzymes. Here we describe how the automated and rapid scaffold search program ScaffoldSelection can be used to find the best starting points, namely protein structures that are most likely to tolerate the introduction and promote the proper formation of a specific catalytic motif.
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Stiel, A.C., Feldmeier, K., Höcker, B. (2014). Identification of Protein Scaffolds for Enzyme Design Using Scaffold Selection. In: Köhler, V. (eds) Protein Design. Methods in Molecular Biology, vol 1216. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1486-9_9
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DOI: https://doi.org/10.1007/978-1-4939-1486-9_9
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