Abstract
The rapidly growing list of small RNA species generated by next-generation sequencing technologies has accelerated the development of new bioinformatics tools for their detection. Small RNAs generated from tRNAs, transfer RNA-derived fragments (tRFs), represent a novel challenge in accurately identifying and distinguishing them from random degradation products of tRNAs. Here, we describe a bioinformatics approach to detect tRFs in next-generation sequencing libraries. We also present a biochemical purification protocol for enriching 5′ tRFs and separating them from miRNAs. And finally, we suggest reliable methods for detecting and quantifying tRFs.
These two authors are equal contributors.
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Acknowledgements
This work was supported by the ARC DP 130103027. G.H. is an ARC Future Fellow.
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Keam, S.P., Sobala, A., Humphreys, D.T., Suter, C.M., Hutvagner, G. (2014). Computational Analysis, Biochemical Purification, and Detection of tRNA-Derived Small RNA Fragments. In: Werner, A. (eds) Animal Endo-SiRNAs. Methods in Molecular Biology, vol 1173. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0931-5_14
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DOI: https://doi.org/10.1007/978-1-4939-0931-5_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0930-8
Online ISBN: 978-1-4939-0931-5
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