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  • © 2014

Animal Endo-SiRNAs

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1173)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Generation of Endo-siRNAs in Xenopus laevis Oocytes

    • Sammer Alnumeir, Andreas Werner
    Pages 27-32
  3. Analysis of Endo-siRNAs in Drosophila

    • Katharina Elmer, Stephanie Helfer, Milijana Mirkovic-Hösle, Klaus Förstemann
    Pages 33-49
  4. Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

    • Do-Hwan Lim, Chun-Taek Oh, Sung-Jun Han, Young Sik Lee
    Pages 51-58
  5. Assays for Direct and Indirect Effects of C. elegans Endo-siRNAs

    • Philip K. Shiu, Jimmy J. Zhuang, Craig P. Hunter
    Pages 71-87
  6. Extraction and Nonradioactive Detection of Small RNA Molecules

    • Mark Carlile, Andreas Werner
    Pages 89-98
  7. p19-Mediated Enrichment and Detection of siRNAs

    • Jingmin Jin, Larry A. McReynolds, Monika Gullerova
    Pages 99-111
  8. Detection of Small Noncoding RNAs by In Situ Hybridization Using Probes of 2′-O-Methyl RNA + LNA

    • Martin Jensen Søe, Martin Dufva, Kim Holmstrøm
    Pages 113-121
  9. Enhanced Detection of Small RNAs Using a Nonradioactive Approach

    • Teresa T. Liu, Zhihua Li, Bino John
    Pages 123-133
  10. Computing siRNA and piRNA Overlap Signatures

    • Christophe Antoniewski
    Pages 135-146
  11. Isolation of Small Interfering RNAs Using Viral Suppressors of RNA Interference

    • Marius van den Beek, Christophe Antoniewski, Clément Carré
    Pages 147-155
  12. Computational Analysis, Biochemical Purification, and Detection of tRNA-Derived Small RNA Fragments

    • Simon P. Keam, Andrew Sobala, David T. Humphreys, Catherine M. Suter, Gyorgy Hutvagner
    Pages 157-167
  13. Back Matter

    Pages 181-182

About this book

Animal Endo-SiRNAs: Methods and Protocols presents a variety of approaches to investigate endo-siRNAs. These include protocols applicable to study short RNAs expressed at a low level and model systems that are particularly suitable to investigate specific aspects of endo-siRNAs, their synthesis, their genomics or regulatory role. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Animal Endo-SiRNAs: Methods and Protocols contains practical tips that are absent in standard lab manuals.

Editors and Affiliations

  • Institute for Cell and Molecular Bioscie, Newcastle University, United Kingdom

    Andreas Werner

Bibliographic Information

  • Book Title: Animal Endo-SiRNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Andreas Werner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-0931-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2014

  • Hardcover ISBN: 978-1-4939-0930-8Published: 12 June 2014

  • Softcover ISBN: 978-1-4939-4160-5Published: 23 August 2016

  • eBook ISBN: 978-1-4939-0931-5Published: 11 June 2014

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 182

  • Number of Illustrations: 12 b/w illustrations, 24 illustrations in colour

  • Topics: Human Genetics

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access