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Quantitative Analysis of Bortezomib-Induced IL-8 Gene Expression in Ovarian Cancer Cells

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Cytokine Bioassays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1172))

Abstract

Interleukin-8 (IL-8), originally discovered as the neutrophil chemoattractant and inducer of leukocyte-mediated inflammation, contributes to cancer progression through its induction of tumor cell proliferation, survival, and migration. IL-8 expression is increased in many types of advanced cancers, including ovarian cancer, and correlates with poor prognosis.

Bortezomib (BZ) is the first FDA-approved proteasome inhibitor that has shown remarkable antitumor activity in multiple myeloma and other hematological malignancies. In solid tumors, including ovarian carcinoma, BZ has been less effective as a single agent; however, the mechanisms remain unknown. We have recently shown that in ovarian cancer cells, BZ greatly increases IL-8 expression, while expression of other NFκB-regulated cytokines, IL-6 and TNF, is unchanged. In this chapter, we describe a protocol that uses real-time qRT-PCR to quantitatively analyze mRNA levels of IL-8 and IL-6 in BZ-treated ovarian cancer cells. The protocol can be easily modified and used for analysis of other cytokines in different cell types.

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Acknowledgement

This work was supported by NIH grant CA173452 to I. Vancurova.

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Authors and Affiliations

  1. Department of Biology, St. John’s University, 8000 Utopia Parkway, Queens, NY, 11439, USA

    Bipradeb Singha, Sai A. Phyo, Himavanth R. Gatla & Ivana Vancurova

Authors
  1. Bipradeb Singha
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  2. Sai A. Phyo
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  3. Himavanth R. Gatla
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  4. Ivana Vancurova
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Corresponding author

Correspondence to Ivana Vancurova .

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Editors and Affiliations

  1. Department of Biological Sciences, St. John's University, New York, New York, USA

    Ivana Vancurova

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Singha, B., Phyo, S.A., Gatla, H.R., Vancurova, I. (2014). Quantitative Analysis of Bortezomib-Induced IL-8 Gene Expression in Ovarian Cancer Cells. In: Vancurova, I. (eds) Cytokine Bioassays. Methods in Molecular Biology, vol 1172. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0928-5_27

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  • DOI: https://doi.org/10.1007/978-1-4939-0928-5_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0927-8

  • Online ISBN: 978-1-4939-0928-5

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