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Cytokine Bioassays

Methods and Protocols

  • Ivana Vancurova

Part of the Methods in Molecular Biology book series (MIMB, volume 1172)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Detection of Released Cytokines by Immunoassays

    1. Front Matter
      Pages 1-1
    2. Haichao Wang, Lin Zhao, Jianhua Li, Shu Zhu, Maggie Yeung
      Pages 13-25
    3. Camilla Heldbjerg Drabe, Thomas Blauenfeldt, Morten Ruhwald
      Pages 27-37
    4. Benoît Liberelle, Charles Fortier, Gregory De Crescenzo
      Pages 39-47
    5. Michael Lee, Abdoullatif Baraket, Nadia Zine, Miguel Zabala, Francesca Campabadal, Raffaele Caruso et al.
      Pages 49-64
    6. Gemma Moncunill, Joseph J. Campo, Carlota Dobaño
      Pages 65-86
  3. Cytokine Bioassays

    1. Front Matter
      Pages 97-97
    2. Dennis Davidson, Hardik Patel
      Pages 107-113
    3. Ved P. Sharma, Brian T. Beaty, Dianne Cox, John S. Condeelis, Robert J. Eddy
      Pages 115-123
    4. Lokesh Sharma, Wenjun Wu, Sanjay L. Dholakiya, Samir Gorasiya, Jiao Wu, Ravikumar Sitapara et al.
      Pages 137-145
    5. Christopher J. Kirk, Saul R. Powell, Edmund J. Miller
      Pages 147-162
    6. Mahmud Arif Pavel, Clarissa Lam, Parul Kashyap, Zahra Salehi-Najafabadi, Gurpreet Singh, Yong Yu
      Pages 185-192
    7. Clarissa Lam, Mahmud Arif Pavel, Parul Kashyap, Zahra Salehi-Najafabadi, Victoria Valentino, Yong Yu
      Pages 193-200
    8. Prajna P. Guha, Sascha A. David, Chandra C. Ghosh
      Pages 201-208
    9. Nicole Salazar, Daniel Muñoz, James Hoy, Bal L. Lokeshwar
      Pages 209-218
  4. Analysis of Cytokine Intracellular Protein Levels and Gene Expression

    1. Front Matter
      Pages 219-219
    2. Jian-Ge Qiu, Xiao-Long Mei, Zhe-Sheng Chen, Zhi Shi
      Pages 235-242
    3. Ling Zhao, Yuan Chou, Yanfang Jiang, Zhenyu Jiang, Cong-Qiu Chu
      Pages 243-256
    4. Himavanth R. Gatla, Bipradeb Singha, Valerie Persaud, Ivana Vancurova
      Pages 271-283
    5. Veronika Miskolci, Louis Hodgson, Dianne Cox, Ivana Vancurova
      Pages 285-293
    6. Bipradeb Singha, Sai A. Phyo, Himavanth R. Gatla, Ivana Vancurova
      Pages 295-304
    7. Felitsiya Shakola, Stephen Byrne, Kainaat Javed, Matteo Ruggiu
      Pages 343-362
  5. Back Matter
    Pages 363-367

About this book

Introduction

Cytokine Bioassays: Methods and Protocols provides a comprehensive collection of classic and cutting-edge methodologies that are used to analyze and quantify cytokines and their biological activities in complex biological and clinical samples. Chapters are divided into three main categories, the first category details the immunodetection of released cytokines in tissue culture supernatants, plasma, serum, and whole blood samples by immunoassays. The second part focuses on the analysis of biologically active cytokines by bioassays using neutralizing antibodies, chemotaxis assay, cytokine-induced phagocytosis assay, proteasome activity assay, and analysis of cytokine-induced immunoglobulin class switching. Part three presents analysis of intracellular cytokines by flow cytometry, immunohistochemistry, immunofluorescence confocal microscopy, and western blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cytokine Bioassays: Methods and Protocols is written by experienced, basic, and clinical researchers with hands-on knowledge of the described protocols. This book will be of interest not only to biochemists, molecular biologists and immunologists but also to physician-scientists working in the field of cytokine research.​

Keywords

Cytokines RT-PCR analysis of intracellular cytokines by flow cytometry biologically active cytokines clinical samples cytokine detection immunodetection of released cytokines immunofluorescence confocal microscopy immunohistochemistry quantitative analysis of cytokine gene expression western blotting

Editors and affiliations

  • Ivana Vancurova
    • 1
  1. 1.Department of Biological SciencesSt. John's UniversityNew YorkUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0928-5
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0927-8
  • Online ISBN 978-1-4939-0928-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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