Abstract
Siderophores are small organic chelators (of molecular weight between 200 and 2,000 Da), having a very high affinity for iron (1017–1043 M−1). They are synthesized by bacteria and secreted into their environment in order to get access to iron, an essential element for bacterial growth. Pyoverdine (also called fluorescins or pseudobactins) and pyochelin are the two major siderophores produced by Pseudomonas aeruginosa in iron-limited media. Methods to specifically detect and measure the amount of pyoverdine and pyochelin in a bacterial culture are provided here. These methods are based on the spectral properties of these two siderophores.
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Acknowledgement
This work was supported by CNRS and ANR grants (ANR-08-BLAN-0315-01 and 05-JCJC-0181-01).
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Hoegy, F., Mislin, G.L.A., Schalk, I.J. (2014). Pyoverdine and Pyochelin Measurements. In: Filloux, A., Ramos, JL. (eds) Pseudomonas Methods and Protocols. Methods in Molecular Biology, vol 1149. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-0473-0_24
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DOI: https://doi.org/10.1007/978-1-4939-0473-0_24
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