Abstract
The baculovirus expression system is an invaluable method for the expression of Herpes Simplex Virus 1 (HSV-1) proteins. The use of insect cells provides a eukaryotic system for the robust expression of heterologous proteins under control of the baculovirus polyhedrin gene promoter that naturally drives the high expression of the polyhedrin protein. Additionally, insect cells often initiate the necessary posttranslational modifications and/or disulfide-bond formation important for the proper folding of the protein. We and others have successfully expressed and purified several HSV-1 proteins including the polymerase, helicase-primase, single-strand DNA binding protein, and alkaline nuclease. The following protocol is based on 15 years of experience from our laboratory.
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Acknowledgments
We thank members of the Weller lab for helpful comments. This work was supported by National Institutes of Health (NIH) grants AI21747 and AI069136 to S.K.W.
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Grady, L.M., Bai, P., Weller, S.K. (2014). HSV-1 Protein Expression Using Recombinant Baculoviruses. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_20
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DOI: https://doi.org/10.1007/978-1-4939-0428-0_20
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