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Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication

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Herpes Simplex Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1144))

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Abstract

The possibility to label specific viral and cellular structures with live cell markers such as autofluorescent proteins has greatly contributed to our understanding of diverse steps of the virus life cycle, as it allows monitoring virus replication in a spatial and temporal fashion. Here, we describe the multi-fluorescent analysis of the multi-compartment herpes simplex virus type-1 by live-cell confocal laser scanning microscopy.

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Reference

  1. De Oliveira AP, Glauser DL, Laimbacher AS et al (2008) Live visualization of herpes simplex virus type 1 compartment dynamics. J Virol 82: 4974–4990

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Authors and Affiliations

  1. Institute of Virology, University of Zurich, Winterthurerstr. 266a, 8057, Zurich, Switzerland

    Michael Seyffert, Cornel Fraefel & Rebecca Vogel

  2. Tumour Virology Division F010, German Cancer Research Center (DKFZ), Heidelberg, Germany

    Anna Paula de Oliveira

Authors
  1. Michael Seyffert
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  2. Anna Paula de Oliveira
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  3. Cornel Fraefel
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  4. Rebecca Vogel
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Corresponding author

Correspondence to Michael Seyffert .

Editor information

Editors and Affiliations

  1. Centre for Virus Research, Westmead Millennium Institute, Westmead, New South Wales, Australia

    Russell J. Diefenbach

  2. Institute of Virology, University of Zürich, Zürich, Switzerland

    Cornel Fraefel

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© 2014 Springer Science+Business Media New York

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Seyffert, M., de Oliveira, A.P., Fraefel, C., Vogel, R. (2014). Multifluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication. In: Diefenbach, R., Fraefel, C. (eds) Herpes Simplex Virus. Methods in Molecular Biology, vol 1144. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0428-0_16

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  • DOI: https://doi.org/10.1007/978-1-4939-0428-0_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0427-3

  • Online ISBN: 978-1-4939-0428-0

  • eBook Packages: Springer Protocols

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