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Caspase-2 Protocols

  • Loretta Dorstyn
  • Sharad Kumar
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1133)

Abstract

Caspase-2 has been shown to function in apoptosis and in some non-apoptotic pathways, including tumor suppression and aging. Caspase-2 has some unique features and is the only caspase that constitutively localizes to the nucleus, although its nuclear function remains unknown. During apoptosis signaling, caspase-2 rapidly homodimerizes, which leads to its activation and proteolytic processing. The activation of caspase-2 can be measured by assessing its dimerization and/or cleavage of the caspase-2 zymogen and its substrates. This chapter outlines commonly used methods to purify recombinant caspase-2 and assess its activity and function in vitro and in cultured cells or tissue extracts.

Key words

Apoptosis Caspase-2 Caspase activity Peptide substrates Recombinant protein Immunoblotting 

Notes

Acknowledgments

The financial support of the National Health and Medical Research Council through project grants 1021456 and 1043057 is gratefully acknowledged. LD is supported by a South Australian Cancer Research Collaborative Fellowship and SK by an NHMRC Senior Principal Research Fellowship (1002863).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Loretta Dorstyn
    • 1
    • 2
  • Sharad Kumar
    • 1
    • 2
  1. 1.Centre for Cancer Biology, SA PathologyAdelaideAustralia
  2. 2.Division of Health SciencesUniversity of South AustraliaAdelaideAustralia

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