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Caspases,Paracaspases, and Metacaspases

Methods and Protocols

  • Peter V. Bozhkov
  • Guy Salvesen

Part of the Methods in Molecular Biology book series (MIMB, volume 1133)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Caspases

    1. Front Matter
      Pages 1-1
    2. Dave Boucher, Catherine Duclos, Jean-Bernard Denault
      Pages 3-39
    3. Marcin Poręba, Aleksandra Szalek, Paulina Kasperkiewicz, Marcin Drąg
      Pages 41-59
    4. Loretta Dorstyn, Sharad Kumar
      Pages 71-87
    5. Mami Yamamoto-Tanaka, Toshihiko Hibino
      Pages 89-100
    6. Eui Seung Lee, Ding Xue
      Pages 101-108
    7. Caitlin E. Fogarty, Andreas Bergmann
      Pages 109-117
    8. Francis McCoy, Rashid Darbandi, Leta K. Nutt
      Pages 119-140
    9. Varsha Kaushal, Christian Herzog, Randy S. Haun, Gur P. Kaushal
      Pages 141-154
    10. Magnus Olsson, Boris Zhivotovsky
      Pages 155-173
  3. Paracaspases and Metacaspases

    1. Front Matter
      Pages 175-175
    2. Stephan Hailfinger, Christiane Pelzer, Margot Thome
      Pages 177-188
    3. Ricardo Martin, Iveth Gonzalez, Nicolas Fasel
      Pages 189-202
    4. Karen McLuskey, Catherine X. Moss, Jeremy C. Mottram
      Pages 203-221
    5. Amit Shrestha, Robin E. C. Lee, Lynn A. Megeney
      Pages 223-235
    6. Elena A. Minina, Simon Stael, Frank Van Breusegem, Peter V. Bozhkov
      Pages 237-253
    7. Liana Tsiatsiani, Simon Stael, Petra Van Damme, Frank Van Breusegem, Kris Gevaert
      Pages 255-261
  4. Back Matter
    Pages 263-266

About this book

Introduction

Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is  a collection of laboratory protocols covering current methods that are employed to measure and detect activities of these proteases in diverse biological systems, ranging from unicellular organisms to mammals.   Broken into two parts, the first part focuses on methods to measure, detect, and inhibit activation and activity of a subset of or specific caspases in vitro and in several model systems and organisms, primarily in the context of programmed cell death.  The second part of the book provides experimental protocols for purification and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Authoritative and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks to aid scientists easy-to-follow techniques.

Keywords

caspase substrate caspases human paracaspase MALT1 in vitro and in vivo analysis of yeast metacaspases paracaspases plant metacaspases programmed cell death proteases protozoan metacaspases purification specific caspases in vitro

Editors and affiliations

  • Peter V. Bozhkov
    • 1
  • Guy Salvesen
    • 2
  1. 1.Dept of Plant Biology & Forest GeneticsSwedish Univ of Agricultural Sciences Linnean Center for Plant BiologyUppsalaSweden
  2. 2.Sanford-Burnham Medical Research Inst. Apoptosis & Cell Death Research PrgmLa JollaUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-0357-3
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0356-6
  • Online ISBN 978-1-4939-0357-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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