Liver Macrophage Isolation by Flow Cytometry Sorting

Part of the Methods in Molecular Biology book series (MIMB, volume 2164)


Liver macrophages are immune cells that under steady state conditions play important roles in maintaining tissue homeostasis. But these cells are also intricately linked to the pathogenesis of obesity-related diseases such as nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and type 2 diabetes (T2D). Described herein is a protocol to isolate liver macrophages by FACS for downstream molecular analysis. Such studies of macrophages are important for elucidating their role in the pathogenesis of these metabolic diseases.

Key words

FACS Isolation Insulin sensitivity Macrophages Liver Obesity Diabetes Inflammation Innate immunity 



Valerio Azzimato and Emelie Barreby are acknowledged for providing cells.


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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Medicine, Integrated Cardio Metabolic CenterKarolinska InstitutetHuddingeSweden

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