Abstract
Liver macrophages are immune cells that under steady state conditions play important roles in maintaining tissue homeostasis. But these cells are also intricately linked to the pathogenesis of obesity-related diseases such as nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and type 2 diabetes (T2D). Described herein is a protocol to isolate liver macrophages by FACS for downstream molecular analysis. Such studies of macrophages are important for elucidating their role in the pathogenesis of these metabolic diseases.
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Valerio Azzimato and Emelie Barreby are acknowledged for providing cells.
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Sulen, A. (2020). Liver Macrophage Isolation by Flow Cytometry Sorting. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_3
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DOI: https://doi.org/10.1007/978-1-0716-0704-6_3
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