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Cellular Assays for Measuring Dynamin Activity in Muscle Cells

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Dynamin Superfamily GTPases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2159))

Abstract

Dynamin is one of the best-studied membrane fission machineries, which mediates endocytic vesicle pinch-off from the plasma membrane. Among the three dynamin isoforms encoded in mammalian genome, dynamin-2 is the ubiquitously expressed isoform and leads to human muscular or neuronal diseases when mutants causing hyperactivity or hypoactivity of its membrane fission activity occur. While transferrin uptake is the most commonly used assay to measure dynamin activity in cultured cells, here we provide two different methods to quantitatively examine the activity of dynamin in myoblasts and myotubes, i.e., Bin1-tubule vesiculation and glucose transporter 4 fractionation assays, respectively. These methods could provide a quantitative measurement of dynamin activity in both differentiated and undifferentiated myoblasts.

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Acknowledgments

This work was supported by Ministry of Science and Technology (MOST) grant 104-2320-B-002-061-MY3 and National Taiwan University grant NTU-CDP-105R7878 to Y.-W. Liu.

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Authors and Affiliations

  1. Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan

    Jessica Laiman & Ya-Wen Liu

  2. Center of Precision Medicine, National Taiwan University, Taipei, Taiwan

    Ya-Wen Liu

Authors
  1. Jessica Laiman
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  2. Ya-Wen Liu
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Corresponding author

Correspondence to Ya-Wen Liu .

Editor information

Editors and Affiliations

  1. School of Medicine, Case Western Reserve University, Cleveland, OH, USA

    Rajesh Ramachandran

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Laiman, J., Liu, YW. (2020). Cellular Assays for Measuring Dynamin Activity in Muscle Cells. In: Ramachandran, R. (eds) Dynamin Superfamily GTPases. Methods in Molecular Biology, vol 2159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0676-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0676-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0675-9

  • Online ISBN: 978-1-0716-0676-6

  • eBook Packages: Springer Protocols

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