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Dynamin Superfamily GTPases

Methods and Protocols

  • Rajesh Ramachandran
Book

Part of the Methods in Molecular Biology book series (MIMB, volume 2159)

Table of contents

  1. Front Matter
    Pages i-x
  2. Yeast DSPs: Isolation and Biochemical Characterization

    1. Front Matter
      Pages 1-1
    2. Nolan W. Kennedy, Lora K. Picton, R. Blake Hill
      Pages 3-15
  3. Mammalian DSPs: Isolation and Biochemical Characterization

    1. Front Matter
      Pages 29-29
    2. Natalia Stepanyants, Patrick J. Macdonald, Pooja Madan Mohan, Rajesh Ramachandran
      Pages 31-40
    3. Ryan W. Clinton, Brianna L. Bauer, Jason A. Mears
      Pages 41-53
    4. Frances Joan D. Alvarez, Peijun Zhang
      Pages 55-65
  4. Biophysical and Cellular Characterization of DSPs

    1. Front Matter
      Pages 83-83
    2. John P. O’Donnell, Carolyn M. Kelly, Holger Sondermann
      Pages 93-113
    3. Nyssa Becker Samanas, Suzanne Hoppins
      Pages 129-140
    4. Pavel V. Bashkirov, Ksenia V. Chekashkina, Anna V. Shnyrova, Vadim A. Frolov
      Pages 141-162
    5. Katherine A. Smith, Emily R. Prantzalos, Arun Anantharam
      Pages 163-178
    6. Jessica Laiman, Ya-Wen Liu
      Pages 179-187
    7. Felipe Montecinos-Franjola, Rajesh Ramachandran
      Pages 205-217
  5. Back Matter
    Pages 219-221

About this book

Introduction

This volume provides methods and protocols on dynamin superfamily GTPases. Chapters are divided into three parts; detailing expression, purification, initial biochemical steps, undescribed analytical techniques, and biophysical and cellular characterization of DSPs in membrane remodeling, fission and fusion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Dynamin Superfamily GTPases: Methods and Protocols to ensure successful results in the further study of this vital field.

Keywords

Dynamin-1 post-translational modifications Mitofusins ATPases NMR

Editors and affiliations

  • Rajesh Ramachandran
    • 1
  1. 1.School of MedicineCase Western Reserve UniversityClevelandUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-0716-0676-6
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0675-9
  • Online ISBN 978-1-0716-0676-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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