Abstract
Tyramide signal amplification (TSA) is an enzyme-mediated method to enhance the immunohistochemical detection of protein, nucleic acid, or other molecules in situ.
Here we describe immunofluorescent detection of a low-abundance cytokine receptor, interleukin-17 receptor B (IL17RB) in U2OS cells, using tyramide signal amplification. In addition, we present a tyramide signal amplification compatible double-color immunostaining protocol using primary antibodies from the same host species. Those applications allow detection of cellular proteins with enhanced sensitivity and add flexibility on primary antibody selection in double- or multicolor immunofluorescence staining assays.
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Acknowledgements
This work was supported by St. John’s University and NIH grant CA213426 to Yan Zhu.
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Wang, H., Pangilinan, R.L., Zhu, Y. (2020). Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence. In: Vancurova, I., Zhu, Y. (eds) Immune Mediators in Cancer. Methods in Molecular Biology, vol 2108. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0247-8_7
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DOI: https://doi.org/10.1007/978-1-0716-0247-8_7
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