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Immune Mediators in Cancer

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Ivana Vancurova

    1. Department of Biological Sciences, St. John’s University, Queens, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Yan Zhu

    1. Department of Biological Sciences, St. John’s University, Queens, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2108)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Detection of Immune Mediators in Cancer Cells and Tissues

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Useful Guide for Analysis of Immune Mediators in Cancer by Fluorochrome (Luminex) Technique

      • Maria Faresjö
      Pages 3-13

    3. Analysis of Circulating HMGB1 in Human Serum

      • Weiqiang Chen, Guoqiang Bao, Lin Zhao, Haichao Wang
      Pages 15-28

    4. Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus

      • Zhuang Ye, Ling Zhao, Qi Gao, Yanfang Jiang, Zhenyu Jiang, Cong-Qiu Chu
      Pages 29-42

    5. Application of Immunohistochemistry in Basic and Clinical Studies

      • Aihua Li, Dong-Hua Yang
      Pages 43-55

    6. Interleukin-1 (IL-1) Immunohistochemistry Assay in Oral Squamous Cell Carcinoma

      • Takaaki Kamatani
      Pages 57-63

    7. Luminex xMAP Assay to Quantify Cytokines in Cancer Patient Serum

      • Helena Kupcova Skalnikova, Katerina Vodickova Kepkova, Petr Vodicka
      Pages 65-88

    8. Detection of Cytokine Receptors Using Tyramide Signal Amplification for Immunofluorescence

      • Herui Wang, Ryan L. Pangilinan, Yan Zhu
      Pages 89-97

  3. Cytokine Bioassays

    1. Front Matter

      Pages 99-99
      Download chapter PDF

    2. Analysis of IFNγ-Induced Migration of Ovarian Cancer Cells

      • Bijaya Gaire, Mohammad M. Uddin, Yue Zou, Ivana Vancurova
      Pages 101-106

    3. Interleukin-8-Induced Invasion Assay in Triple-Negative Breast Cancer Cells

      • Mohammad M. Uddin, Bijaya Gaire, Betsy Deza, Ivana Vancurova
      Pages 107-115

    4. Interleukin-8 Induces Proliferation of Ovarian Cancer Cells in 3D Spheroids

      • Mohammad M. Uddin, Bijaya Gaire, Ivana Vancurova
      Pages 117-124

    5. Detection of Ferroptosis by BODIPY™ 581/591 C11

      • Alejandra M. Martinez, Ahryun Kim, Wan Seok Yang
      Pages 125-130

    6. Methods for Studying TNFα-Induced Autophagy

      • Sheyda Najafi, Ehab M. Abo-Ali, Vikas V. Dukhande
      Pages 131-146

    7. Isolation of Antibody Binders to MISIIR from a Phage Display Library by Sorting

      • Andy Qingan Yuan
      Pages 147-157

    8. Measuring Chimeric Antigen Receptor T Cells (CAR T Cells) Activation by Coupling Intracellular Cytokine Staining with Flow Cytometry

      • Chong Xu, Yibo Yin
      Pages 159-165

    9. Analysis of Interleukin-4-Induced Class Switch Recombination in Mouse Myeloma CH12F3-2 Cells

      • Wenjun Wu, Zhihui Xiao, Deon Buritis, Vladimir Poltoratsky
      Pages 167-179

    10. Single-Stranded DNA Aptamers Against TNF and Their Potential Applications

      • Shao Tao, Pingfang Song, Xiaowei Zhang, Lingshu Zhang, Cong-Qiu Chu
      Pages 181-196

    11. Probing Metabolic Changes in IFNγ-Treated Ovarian Cancer Cells

      • Pritpal Kaur, Shreya Nagar, Madhura Bhagwat, Mohammad M. Uddin, Yan Zhu, Ales Vancura
      Pages 197-207
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Keywords

About this book

This book provides a comprehensive collection of classic and cutting-edge methodologies as well as bioinformatics and genome-editing approaches that are used to quantify immune mediators and analyze their function and biological activity in cancer cells and tissues. Beginning with a section on the detection of immune mediators in samples, the volume continues with sections covering cytokine bioassays, the expression and regulation of immune mediators in cancer cells, and methods to navigate the enormous datasets created by modern DNA and RNA sequencing and proteomic technology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and wide-ranging, Immune Mediators in Cancer: Methods and Protocols serves as a valuable resource for biochemists, molecular biologists, cancer biologists, and immunologists, as well as for physician-scientists working in the field of immunology and cancer research.

Editors and Affiliations

  • Department of Biological Sciences, St. John’s University, Queens, USA

    Ivana Vancurova, Yan Zhu

Bibliographic Information

  • Book Title: Immune Mediators in Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Ivana Vancurova, Yan Zhu

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0247-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0246-1Published: 15 January 2020

  • Softcover ISBN: 978-1-0716-0249-2Published: 15 January 2021

  • eBook ISBN: 978-1-0716-0247-8Published: 14 January 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 373

  • Number of Illustrations: 11 b/w illustrations, 70 illustrations in colour

  • Topics: Cancer Research, Immunology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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