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Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus

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Immune Mediators in Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2108))

Abstract

Interleukin (IL)-22 belongs to the IL-10 family of cytokines. IL-22 exerts its biological effects via members of the cytokine receptor family class 2. CD4+ T helper (Th) cells predominantly producing IL-22 have been designated as Th22 cells. IL-22/Th22 cells are functionally related to IL-17/Th17 cells, but are distinctly different. Both IL-22 and IL-17 are cytokines recruiting neutrophils in response to microbe invasion. In chronic inflammation, IL-22 mediates protective and regenerative processes, whereas IL-17 cytokines tend to induce inflammation. Studies found that increased IL-22 levels and Th22 cells in peripheral blood were associated with disease activity in patients with systemic lupus erythematosus (SLE), but decreased IL-22 and Th22 cells were also reported. Here we describe analysis of IL-22 and Th22 cells in peripheral blood quantified by flow cytometry, and correlate our findings with SLE disease activity.

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References

  1. Renauld JC (2003) Class II cytokine receptors and their ligands: key antiviral and inflammatory modulators. Nat Rev Immunol 3(8):667–676

    Article  CAS  Google Scholar 

  2. Dumoutier L, Lejeune D, Colau D, Renauld JC (2001) Cloning and characterization of IL-22 binding protein, a natural antagonist of IL-10-related T cell-derived inducible factor/IL-22. J Immunol 166(12):7090–7095

    Article  CAS  Google Scholar 

  3. Gruenberg BH, Schoenemeyer A, Weiss B, Toschi L, Kunz S, Wolk K, Asadullah K, Sabat R (2001) A novel, soluble homologue of the human IL-10 receptor with preferential expression in placenta. Genes Immun 2(6):329–334

    Article  CAS  Google Scholar 

  4. Wei CC, Ho TW, Liang WG, Chen GY, Chang MS (2003) Cloning and characterization of mouse IL-22 binding protein. Genes Immun 4(3):204–211

    Article  CAS  Google Scholar 

  5. Valeri M, Raffatellu M (2016) Cytokines IL-17 and IL-22 in the host response to infection. Pathog Dis 74(9):ftw111

    Article  Google Scholar 

  6. McAleer JP, Kolls JK (2014) Directing traffic: IL-17 and IL-22 coordinate pulmonary immune defense. Immunol Rev 260(1):129–144

    Article  CAS  Google Scholar 

  7. Duhen T, Geiger R, Jarrossay D, Lanzavecchia A, Sallusto F (2009) Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat Immunol 10(8):857–863

    Article  CAS  Google Scholar 

  8. Trifari S, Kaplan CD, Tran EH, Crellin NK, Spits H (2009) Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells. Nat Immunol 10(8):864–871

    Article  CAS  Google Scholar 

  9. Eyerich S, Eyerich K, Pennino D, Carbone T, Nasorri F, Pallotta S et al (2009) Th22 cells represent a distinct human T cell subset involved in epidermal immunity and remodeling. J Clin Invest 119(12):3573–3585

    CAS  PubMed  PubMed Central  Google Scholar 

  10. Eyerich K, Eyerich S (2015) Th22 cells in allergic disease. Allergo J Int 24(1):1–7

    Article  Google Scholar 

  11. Fujita H, Nograles KE, Kikuchi T, Gonzalez J, Carucci JA, Krueger JG (2009) Human Langerhans cells induce distinct IL-22-producing CD4+ T cells lacking IL-17 production. Proc Natl Acad Sci U S A 106(51):21795–21800

    Article  CAS  Google Scholar 

  12. Moy AP, Murali M, Kroshinsky D, Duncan LM, Nazarian RM (2015) Immunologic overlap of helper T-cell subtypes 17 and 22 in erythrodermic psoriasis and atopic dermatitis. JAMA Dermatol 151(7):753–760

    Article  Google Scholar 

  13. Moy AP, Murali M, Nazarian RM (2016) Identification of a Th2- and Th17-skewed immune phenotype in chronic urticaria with Th22 reduction dependent on autoimmunity and thyroid disease markers. J Cutan Pathol 43(4):372–378

    Article  Google Scholar 

  14. Volpe E, Servant N, Zollinger R, Bogiatzi SI, Hupe P, Barillot E, Soumelis V (2008) A critical function for transforming growth factor-beta, interleukin 23 and proinflammatory cytokines in driving and modulating human T(H)-17 responses. Nat Immunol 9(6):650–657

    Article  CAS  Google Scholar 

  15. Acosta-Rodriguez EV, Rivino L, Geginat J, Jarrossay D, Gattorno M, Lanzavecchia A et al (2007) Surface phenotype and antigenic specificity of human interleukin 17-producing T helper memory cells. Nat Immunol 8(6):639–646

    Article  CAS  Google Scholar 

  16. Kaul A, Gordon C, Crow MK, Touma Z, Urowitz MB, van Vollenhoven R, Ruiz-Irastorza G, Hughes G (2016) Systemic lupus erythematosus. Nat Rev Dis Primers 22:16039

    Article  Google Scholar 

  17. Zhao L, Jiang Z, Jiang Y, Ma N, Wang K, Zhang Y, Feng L (2013) IL-22+CD4+ T-cells in patients with active systemic lupus erythematosus. Exp Biol Med (Maywood) 238(2):193–199

    Article  CAS  Google Scholar 

  18. Zhao L, Ma H, Jiang Z, Jiang Y, Ma N (2014) Immunoregulation therapy changes the frequency of interleukin (IL)-22+ CD4+ T cells in systemic lupus erythematosus patients. Clin Exp Immunol 177(1):212–218

    Article  CAS  Google Scholar 

  19. Zhong W, Jiang Y, Ma H, Wu J, Jiang Z, Zhao L (2017) Elevated levels of CCR6(+) T helper 22 cells correlate with skin and renal impairment in systemic lupus erythematosus. Sci Rep 7(1):12962

    Article  Google Scholar 

  20. Yang XY, Wang HY, Zhao XY, Wang LJ, Lv QH, Wang QQ (2013) Th22, but not Th17 might be a good index to predict the tissue involvement of systemic lupus erythematosus. J Clin Immunol 33(4):767–774

    Article  CAS  Google Scholar 

  21. Lin J, Yue LH, Chen WQ (2014) Decreased plasma IL-22 levels and correlations with IL-22-producing T helper cells in patients with new-onset systemic lupus erythematosus. Scand J Immunol 79(2):131–136

    Article  CAS  Google Scholar 

  22. Pan HF, Zhao XF, Yuan H, Zhang WH, Li XP, Wang GH et al (2009) Decreased serum IL-22 levels in patients with systemic lupus erythematosus. Clin Chim Acta 401(1–2):179–180

    Article  CAS  Google Scholar 

  23. Wang R, Zeng YL, Qin HM, Lu YL, Huang HT, Lei M et al (2018) Association of interleukin 22 gene polymorphisms and serum IL-22 level with risk of systemic lupus erythematosus in a Chinese population. Clin Exp Immunol 193(2):143–151

    Article  CAS  Google Scholar 

  24. Yang X, Weng Q, Hu L, Yang L, Wang X, Xiang X, Hong B, Gong X, Wang Q (2019) Increased interleukin-22 levels in lupus nephritis and its associated with disease severity: a study in both patients and lupus-like mice model. Clin Exp Rheumatol 37(3):400–407

    PubMed  Google Scholar 

  25. Rekik R, Smiti Khanfir M, Larbi T, Zamali I, Beldi-Ferchiou A, Kammoun O et al (2018) Impaired TGF-beta signaling in patients with active systemic lupus erythematosus is associated with an overexpression of IL-22. Cytokine 108:182–189

    Article  CAS  Google Scholar 

  26. Zhao L, Chou Y, Jiang Y, Jiang Z, Chu CQ (2014) Analysis of IL-17 production by flow cytometry and ELISPOT assays. Methods Mol Biol 1172:243–256

    Article  Google Scholar 

  27. Bombardier C, Gladman DD, Urowitz MB, Caron D, Chang CH (1992) Derivation of the SLEDAI. A disease activity index for lupus patients. The Committee on Prognosis Studies in SLE. Arthritis Rheum 35(6):630–640

    Article  CAS  Google Scholar 

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 81501343), Bethune Plan Project of Jilin University (2015410). CQC was supported by Rheumatology Research Foundation Innovative and Pilot grants.

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Authors and Affiliations

  1. Department of Rheumatology and Immunology, The First Bethune Hospital, Jilin University, Changchun, China

    Zhuang Ye, Ling Zhao, Qi Gao & Zhenyu Jiang

  2. Department of Central Laboratory, The First Bethune Hospital, Jilin University, Changchun, China

    Yanfang Jiang

  3. Division of Arthritis and Rheumatic Diseases, Oregon Health & Science University, Portland, OR, USA

    Cong-Qiu Chu

  4. Section of Rheumatology, VA Portland Health Care System, Portland, OR, USA

    Cong-Qiu Chu

Authors
  1. Zhuang Ye
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  2. Ling Zhao
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  3. Qi Gao
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  4. Yanfang Jiang
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  5. Zhenyu Jiang
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  6. Cong-Qiu Chu
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Corresponding author

Correspondence to Cong-Qiu Chu .

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Editors and Affiliations

  1. Department of Biological Sciences, St. John’s University, Queens, NY, USA

    Ivana Vancurova

  2. Department of Biological Sciences, St. John’s University, Queens, NY, USA

    Yan Zhu

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Ye, Z., Zhao, L., Gao, Q., Jiang, Y., Jiang, Z., Chu, CQ. (2020). Analysis of IL-22 and Th22 Cells by Flow Cytometry in Systemic Lupus Erythematosus. In: Vancurova, I., Zhu, Y. (eds) Immune Mediators in Cancer. Methods in Molecular Biology, vol 2108. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0247-8_3

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  • DOI: https://doi.org/10.1007/978-1-0716-0247-8_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0246-1

  • Online ISBN: 978-1-0716-0247-8

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