Abstract
Induced pluripotent stem cells from nonhuman primates (NHPs) have unique roles in cell biology and regenerative medicine. Because of the relatedness of NHPs to humans, NHP iPS cells can serve as a source of differentiated derivatives that can be used to address important questions in the comparative biology of primates. Additionally, when used as a source of cells for regenerative medicine, NHP iPS cells serve an invaluable role in translational experiments in cell therapy. Reprogramming of NHP somatic cells requires the same conditions as previously established for human cells. However, throughout the process, a variety of modifications to the human cell protocols must be made to accommodate significant species differences.
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References
Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663–676
Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872
Liu H, Zhu F, Yong J, Zhang P, Hou P, Li H, Jiang W, Cai J, Liu M, Cui K, Qu X, Xiang T, Lu D, Chi X, Gao G, Ji W, Ding M, Deng H (2008) Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts. Cell Stem Cell 3:587–590
Wu Y, Zhang Y, Mishra A, Tardif SD, Hornsby PJ (2010) Generation of induced pluripotent stem cells from newborn marmoset skin fibroblasts. Stem Cell Res 4:180–188
Tomioka I, Maeda T, Shimada H, Kawai K, Okada Y, Igarashi H, Oiwa R, Iwasaki T, Aoki M, Kimura T, Shiozawa S, Shinohara H, Suemizu H, Sasaki E, Okano H (2010) Generating induced pluripotent stem cells from common marmoset (Callithrix jacchus) fetal liver cells using defined factors, including Lin28. Genes Cells 15:959–969
Ludwig TE, Levenstein ME, Jones JM, Berggren WT, Mitchen ER, Frane JL, Crandall LJ, Daigh CA, Conard KR, Piekarczyk MS, Llanas RA, Thomson JA (2006) Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol 24:185–187
Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods 8:424–429
Wu Y, Melton DW, Zhang Y, Hornsby PJ (2009) Improved coinfection with amphotropic pseudotyped retroviral vectors. J Biomed Biotechnol 2009:901079
Morita S, Kojima T, Kitamura T (2000) Plat-E: an efficient and stable system for transient packaging of retroviruses. Gene Ther 7:1063–1066
Huangfu D, Osafune K, Maehr R, Guo W, Eijkelenboom A, Chen S, Muhlestein W, Melton DA (2008) Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat Biotechnol 26:1269–1275
Polo JM, Hochedlinger K (2010) When fibroblasts MET iPSCs. Cell Stem Cell 7:5–6
Chen KG, Mallon BS, Hamilton RS, Kozhich OA, Park K, Hoeppner DJ, Robey PG, McKay RD (2012) Non-colony type monolayer culture of human embryonic stem cells. Stem Cell Res 9:237–248
Valbuena D, Sanchez-Luengo S, Galan A, Sanchez E, Gomez E, Poo ME, Ruiz V, Genbacev O, Krtolica A, Pellicer A, Moreno R, Simon C (2008) Efficient method for slow cryopreservation of human embryonic stem cells in xeno-free conditions. Reprod Biomed Online 17:127–135
Koch CA, Jordan CE, Platt JL (2006) Complement-dependent control of teratoma formation by embryonic stem cells. J Immunol 177:4803–4809
Papaioannou VE, Fox JG (1993) Efficacy of tribromoethanol anesthesia in mice. Lab Anim Sci 43:189–192
Wu Y, Mishra A, Qiu Z, Farnsworth S, Tardif SD, Hornsby PJ (2012) Nonhuman primate induced pluripotent stem cells in regenerative medicine. Stem Cells Int 2012:767195
Lu J, Liu H, Huang CT, Chen H, Du Z, Liu Y, Sherafat MA, Zhang SC (2013) Generation of integration-free and region-specific neural progenitors from primate fibroblasts. Cell Rep 3:1580–1591
Yamaguchi S, Marumoto T, Nii T, Kawano H, Liao J, Nagai Y, Okada M, Takahashi A, Inoue H, Sasaki E, Fujii H, Okano S, Ebise H, Sato T, Suyama M, Okano H, Miura Y, Tani K (2014) Characterization of common marmoset dysgerminoma-like tumor induced by the lentiviral expression of reprogramming factors. Cancer Sci 105:402–408
Adams SR, Muchmore E, Richardson JH (1995) Biosafety. In: Bennett BT, Abee CR, Henrickson RV (eds) Nonhuman primates in biomedical research: biology and management. Academic Press, San Diego, pp 377–420
Beers J, Gulbranson DR, George N, Siniscalchi LI, Jones J, Thomson JA, Chen G (2012) Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions. Nat Protoc 7:2029–2040
Dolgin E (2010) Putting stem cells to the test. Nat Med 16:1354–1357
Acknowledgments
This work was supported by VA grant I01BX001454, NIH grants R21 AG033286 and R03 AG045481, and by grants from the Owens Medical Research Foundation and the Ted Nash Long Life Foundation.
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Mishra, A. et al. (2014). Induced Pluripotent Stem Cells from Nonhuman Primates. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_159
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DOI: https://doi.org/10.1007/7651_2014_159
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