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  • © 2016

Induced Pluripotent Stem (iPS) Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1357)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xv
  2. Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

    • Ruijun Jeanna Su, Amanda Neises, Xiao-Bing Zhang
    Pages 57-69
  3. Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells by Plasmid Transfection

    • Jong Soo Kim, Hyun Woo Choi, Yean Ju Hong, Jeong Tae Do
    Pages 85-95
  4. Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons

    • Shin-Il Kim, Fabian Oceguera-Yanez, Chiho Sakurai, Masato Nakagawa, Shinya Yamanaka, Knut Woltjen
    Pages 111-131
  5. Generation and Characterization of Rat iPSCs

    • Jing Liao, Chun Cui
    Pages 133-148
  6. Generation of Induced Pluripotent Stem Cells in Rabbits

    • Marielle Afanassieff, Yann Tapponnier, Pierre Savatier
    Pages 149-172
  7. Induced Pluripotent Stem Cells from Nonhuman Primates

    • Anuja Mishra, Zhifang Qiu, Steven L. Farnsworth, Jacob J. Hemmi, Miao Li, Alexander V. Pickering et al.
    Pages 183-193
  8. cGMP-Compliant Expansion of Human iPSC Cultures as Adherent Monolayers

    • Ann M. Parr, Patrick J. Walsh, Vincent Truong, James R. Dutton
    Pages 221-229
  9. Analysis of the Mitochondrial DNA and Its Replicative Capacity in Induced Pluripotent Stem Cells

    • Gael Cagnone, Vijesh Vaghjiani, William Lee, Claire Sun, Jacqueline Johnson, Ka-Yu Yeung et al.
    Pages 231-267
  10. Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell-Derived Progenitor Cell Lines

    • Paola A. Bignone, Rachel A. Krupa, Michael D. West, David Larocca
    Pages 269-283
  11. Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells

    • Ayaka Yanagida, Hiromitsu Nakauchi, Akihide Kamiya
    Pages 295-310

About this book

This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocols guides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.

 

Editors and Affiliations

  • Ottawa Hospital Research Institute, Ottawa, Canada

    Kursad Turksen

  • Mount Sinai Hospital Samuel Lunenfeld Research Inst, Toronto, Canada

    Andras Nagy

Bibliographic Information

  • Book Title: Induced Pluripotent Stem (iPS) Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen, Andras Nagy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-3055-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2016

  • Hardcover ISBN: 978-1-4939-3054-8Published: 29 December 2015

  • Softcover ISBN: 978-1-4939-7981-3Published: 27 March 2019

  • eBook ISBN: 978-1-4939-3055-5Published: 28 December 2015

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 496

  • Number of Illustrations: 32 b/w illustrations, 44 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access