Abstract
Induced pluripotent stem (iPS) cells that are potentially similar to embryonic stem (ES) cells can be artificially established by introduction into somatic cells of the transgenes POU5F1 (also known as Oct3/4), SOX2, KLF4, and c-MYC. In cynomolgus monkeys (Macaca fascicularis), iPS cells generated by using these four allogeneic transgenes should be an important resource for various types of biomedical research because the use of xenogeneic transgenes may cause complications. To establish such iPS cells, cynomolgus monkey somatic cells were infected with amphotropic retroviral vectors, which were derived from Plat-A cells, containing cDNA for the cynomolgus monkey genes POU5F1, SOX2, KLF4, and c-MYC. As a result, iPS cells could be established from somatic cells from fetal liver and newborn skin of cynomolgus monkeys, similarly to the case for mouse and human somatic cells.
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Acknowledgements
I thank Ryoichi Ono, Manami Shimada, Hiroaki Shibata, Ichiro Takahashi, Hiroyasu Inada, Tatsuyuki Takada, Tetsuya Nosaka, and Yasuhiro Yasutomi for this experiment. I am also grateful to Prof. Toshio Kitamura for providing pMXs retroviral vectors and Plat-A cells.
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Shimozawa, N. (2014). Cynomolgus Monkey Induced Pluripotent Stem Cells Generated By Using Allogeneic Genes. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2014_137
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DOI: https://doi.org/10.1007/7651_2014_137
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3054-8
Online ISBN: 978-1-4939-3055-5
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