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Isolation and Characterisation of Human Adipose-Derived Stem Cells

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Immunological Tolerance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1899))

Abstract

Recently, adipose-derived stem cells (ASCs), obtained from fresh human lipoaspirate, have shown promise as immunomodulatory agents having demonstrated immunosuppressive functionality both in vitro and in vivo. A number of researchers have described the isolation of ASCs through the enzymatic digestion of fat samples, followed by a number of purification steps, involving centrifugation and filtration. Here, we utilize a standard isolation technique, with the added purification of putative ASCs by fluorescence activated cell sorting (FACS). ASCs are an extremely valuable resource in clinical applications, including reconstruction, regeneration, and investigations into immune activity. This method could be used to isolate and purify ASCs for such downstream applications.

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Acknowledgments

This work was supported by the Restoration of Appearance and Function Trust (RAFT), with funding from the Kirby Laing Foundation.

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Authors and Affiliations

  1. Research Department of Surgical Biotechnology, UCL Division of Surgery and Interventional Science, University College London, Royal Free Hospital, London, UK

    Anna Wilson, Melisse Chee & Peter Butler

  2. Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology Division of Surgery and Interventional Science University College London (UCL), Royal Free Hospital, London, UK

    Ashleigh S. Boyd

  3. UCL Institute of Immunity and Transplantation, Royal Free Hospital, London, UK

    Ashleigh S. Boyd

Authors
  1. Anna Wilson
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  2. Melisse Chee
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  3. Peter Butler
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  4. Ashleigh S. Boyd
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Corresponding author

Correspondence to Ashleigh S. Boyd .

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Editors and Affiliations

  1. Associate Professor in Stem Cell Biology and Regenerative Medicine, Research Department of Surgical Biotechnology, Division of Surgery and Interventional Science, University College London (UCL), Royal Free Hospital, London, UK

    Ashleigh S. Boyd

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Wilson, A., Chee, M., Butler, P., Boyd, A.S. (2019). Isolation and Characterisation of Human Adipose-Derived Stem Cells. In: Boyd, A. (eds) Immunological Tolerance. Methods in Molecular Biology, vol 1899. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8938-6_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8938-6_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8936-2

  • Online ISBN: 978-1-4939-8938-6

  • eBook Packages: Springer Protocols

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