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Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level

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Glucose Transport

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1713))

Abstract

Microfluidic systems in combination with microscopy (e.g., fluorescence) can be a powerful tool to study, at single-cell level, the behavior and morphology of biological cells after uptake of glucose. Here, we briefly discuss the advantages of using microfluidic systems. We further describe how microfluidic systems are fabricated and how they are utilized. Finally, we discuss how the large amount of data can be analyzed in a “semi-automatic” manner using custom-made software. In summary, we provide a guide to how to use microfluidic systems in single-cell studies.

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Acknowledgements

We wish to acknowledge the Science Faculty of the University of Gothenburg for financing and supporting the PDMS Microfluidic Fabrication Facility.

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Authors and Affiliations

  1. Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden

    Niek Welkenhuysen & Stefan Hohmann

  2. Department of Physics, University of Gothenburg, Gothenburg, Sweden

    Caroline B. Adiels & Mattias Goksör

  3. Department of Biology and Biological Engineering, Systems and Synthetic Biology, Chalmers University of Technology, Kemivägen 10, SE-412 96, Gothenburg, Sweden

    Stefan Hohmann

Authors
  1. Niek Welkenhuysen
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  2. Caroline B. Adiels
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  3. Mattias Goksör
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  4. Stefan Hohmann
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Corresponding author

Correspondence to Stefan Hohmann .

Editor information

Editors and Affiliations

  1. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Karin Lindkvist-Petersson

  2. Department of Experimental Medical Science, Lund University, Lund, Sweden

    Jesper S Hansen

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Welkenhuysen, N., Adiels, C.B., Goksör, M., Hohmann, S. (2018). Applying Microfluidic Systems to Study Effects of Glucose at Single-Cell Level. In: Lindkvist-Petersson, K., Hansen, J. (eds) Glucose Transport. Methods in Molecular Biology, vol 1713. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7507-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-7507-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7506-8

  • Online ISBN: 978-1-4939-7507-5

  • eBook Packages: Springer Protocols

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