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Sample Preparation for Proteomic Analysis of Neisseria meningitidis

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Neisseria meningitidis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1969))

Abstract

Mass spectrometry (MS) proteomics allows systematic identification, characterization, and relative quantification of the full suite of proteins in a biological sample, and is a powerful analytical approach for investigation of many aspects of the biology of Neisseria meningitidis. Here, we describe methods for robust and efficient sample preparation of the proteome of N. meningitidis suitable for diverse MS proteomics workflows.

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Acknowledgments

This work was supported by National Health and Medical Research Council Career Development Fellowship APP1087975 to B.L.S.

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Authors and Affiliations

  1. School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, Australia

    Benjamin L. Schulz

  2. Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, QLD, Australia

    Benjamin L. Schulz

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  1. Benjamin L. Schulz
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Corresponding author

Correspondence to Benjamin L. Schulz .

Editor information

Editors and Affiliations

  1. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Kate L. Seib

  2. Institute For Glycomics, Griffith University, Southport, QLD, Australia

    Ian R. Peak

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Schulz, B.L. (2019). Sample Preparation for Proteomic Analysis of Neisseria meningitidis. In: Seib, K., Peak, I. (eds) Neisseria meningitidis. Methods in Molecular Biology, vol 1969. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9202-7_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9202-7_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9201-0

  • Online ISBN: 978-1-4939-9202-7

  • eBook Packages: Springer Protocols

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