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Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains

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Astrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1938))

Abstract

Astrocytes play fundamental roles in development and plasticity of the central nervous system. Dysfunction of astrocytes contributes to most of neurological diseases ranging from neurodegenerative diseases to psychological disorders. To better understand the involvement of astrocytes in both physiological and pathological conditions, and further elucidate their underlying mechanisms, it is critical to establish a reliable isolation and culture method for the cells. In this chapter, we describe the isolation and culture protocols of astrocytes from postnatal and adult mouse brains.

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Authors and Affiliations

  1. Neuroscience Drug Discovery, Janssen Research and Development, LLC, San Diego, CA, USA

    Yingbo He, Natalie Taylor & Anindya Bhattacharya

Authors
  1. Yingbo He
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  2. Natalie Taylor
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  3. Anindya Bhattacharya
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Corresponding author

Correspondence to Yingbo He .

Editor information

Editors and Affiliations

  1. Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany

    Barbara Di Benedetto

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He, Y., Taylor, N., Bhattacharya, A. (2019). Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains. In: Di Benedetto, B. (eds) Astrocytes. Methods in Molecular Biology, vol 1938. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9068-9_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9068-9_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9067-2

  • Online ISBN: 978-1-4939-9068-9

  • eBook Packages: Springer Protocols

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