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Astrocytes

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Barbara Di Benedetto

    1. Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1938)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
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  2. Astrocyte Biology: Overviews and Perspectives

    1. Front Matter

      Pages 1-1
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    2. The Astrocyte–Neuron Interface: An Overview on Molecular and Cellular Dynamics Controlling Formation and Maintenance of the Tripartite Synapse

      • Uzma Hasan, Sandeep K. Singh
      Pages 3-18

    3. Role of Astrocytes in the Neurogenic Niches

      • Julia Schneider, Julian Karpf, Ruth Beckervordersandforth
      Pages 19-33

  3. Isolation of Astrocytes from Animal Models

    1. Front Matter

      Pages 35-35
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    2. Isolation and Culture of Astrocytes from Postnatal and Adult Mouse Brains

      • Yingbo He, Natalie Taylor, Anindya Bhattacharya
      Pages 37-47

    3. Fluorescence-Activated Cell Sorting-Based Isolation and Characterization of Neural Stem Cells from the Adult Zebrafish Telencephalon

      • Rossella Di Giaimo, Sven Aschenbroich, Jovica Ninkovic
      Pages 49-66

  4. Astrocyte Morphology and Functions: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro

    1. Front Matter

      Pages 67-67
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    2. A Method to Visualize the Nanoscopic Morphology of Astrocytes In Vitro and In Situ

      • Janosch P. Heller, Dmitri A. Rusakov
      Pages 69-84

    3. A Specialized Method to Resolve Fine 3D Features of Astrocytes in Nonhuman Primate (Marmoset, Callithrix jacchus) and Human Fixed Brain Samples

      • Gaël Quesseveur, Aymeric Fouquier d’Hérouël, Keith K. Murai, David S. Bouvier
      Pages 85-95

    4. Cisterna Magna Injection in Rats to Study Glymphatic Function

      • Marta Ramos, Nicholas Burdon Bechet, Roberta Battistella, Chiara Pavan, Anna L. R. Xavier, Maiken Nedergaard et al.
      Pages 97-104

    5. Preparing the Astrocyte Perivascular Endfeet Transcriptome to Investigate Astrocyte Molecular Regulations at the Brain–Vascular Interface

      • Anne-Cécile Boulay, Noémie Mazaré, Bruno Saubaméa, Martine Cohen-Salmon
      Pages 105-116

    6. Monitoring Interneuron–Astrocyte Signaling and Its Consequences on Synaptic Transmission

      • Sara Mederos, Gertrudis Perea
      Pages 117-129

    7. Astrocytes and Circadian Rhythms: An Emerging Astrocyte–Neuron Synergy in the Timekeeping System

      • Olga Barca Mayo, Luca Berdondini, Davide De Pietri Tonelli
      Pages 131-154

    8. In Vitro Engulfment Assay to Measure Phagocytic Activity of Astrocytes Using Synaptosomes

      • Youkyeong Gloria Byun, Won-Suk Chung
      Pages 155-168

    9. Quantifying Compartment-Specific Protein Translocation in Astrocytes by Object-Oriented Image Analysis: Mitochondrial Translocation of PKCδ

      • Kiavasch Mohammad Nejad Farid, Amin Derouiche
      Pages 169-186

    10. Imaging of Local and Global Sodium Signals in Astrocytes

      • Niklas J. Gerkau, Karl W. Kafitz, Christine R. Rose
      Pages 187-202

    11. Detection of Protein Uptake in In Vitro Cultured Astrocytes Exemplified by the Uptake of the Serine Protease, Tissue Plasminogen Activator

      • Ewa E. Bres, Andreas Faissner
      Pages 203-217

    12. Native Chromatin Immunoprecipitation (N-ChIP) in Primary Cortical Rat Astrocytes

      • Victoria Adelheid Malik, Barbara Di Benedetto, Mira Jakovcevski
      Pages 219-229

  5. Astrocyte Pathologies in the Central Nervous System: Emerging Methods for Their Characterizations In Vivo, In Situ, and In Vitro

    1. Front Matter

      Pages 231-231
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Keywords

About this book

This detailed volume gathers together a broad variety of methods essential to the investigation of the biology of astrocytes and their multifaceted roles in both healthy and diseased brains. Beginning with some overviews of the subject, the book continues by covering techniques for the isolation of astrocytes from animal models, the investigation of astrocyte morphology and function, as well as for understanding astrocyte pathologies in the central nervous system. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Astrocytes: Methods and Protocols serves as an ideal guide for both experienced and beginner scientists working toward unraveling the novel, fascinating roles of these versatile cells.

Editors and Affiliations

  • Department of Psychiatry and Psychotherapy, University of Regensburg, Regensburg, Germany

    Barbara Di Benedetto

Bibliographic Information

  • Book Title: Astrocytes

  • Book Subtitle: Methods and Protocols

  • Editors: Barbara Di Benedetto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9068-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9067-2Published: 08 January 2019

  • eBook ISBN: 978-1-4939-9068-9Published: 08 January 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 295

  • Number of Illustrations: 6 b/w illustrations, 60 illustrations in colour

  • Topics: Neurosciences, Cell Biology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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