Abstract
Bacteria that escape from membrane-enclosed vacuoles to the cytosol of cells are targeted by autophagy, which recognizes and captures bacteria into autophagosomes wherein their proliferation is restricted. Here we discuss two means by which antibacterial autophagy is assessed: (1) the visualization and enumeration of autophagy protein recruitment to the vicinity of cytosolic bacteria by means of immunofluorescence microscopy and (2) the measurement of autophagy-dependent restriction of bacterial proliferation by means of colony-forming unit assay.
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Acknowledgments
This work was supported by the MRC (U105170648) and the Wellcome Trust (WT104752MA).
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Boyle, K.B., Randow, F. (2019). Measuring Antibacterial Autophagy. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_45
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DOI: https://doi.org/10.1007/978-1-4939-8873-0_45
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