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Methods to Determine the Role of Autophagy Proteins in C. elegans Aging

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Autophagy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1880))

Abstract

This chapter describes methods for the analysis of autophagy proteins in C. elegans aging. We discuss the strains to be considered, the methods for the delivery of double-stranded RNA, and the methods to measure autophagy levels, autophagic flux, and degradation by autophagy.

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Acknowledgments

Work in the Henis-Korenblit laboratory is supported by grants: no. 2013188 from the Binational Science Foundation, no. I-1211-309.13/2012 from the German-Israeli Foundation for Scientific Research and Development, and no. 3-12066 from the Israeli Ministry of Science, Technology and Space, and work in the Meléndez Lab is supported by grants R15GM102846 from the National Institute of Health, a PSC-CUNY Research award, and A.M. was an Ellison Medical Foundation New Scholar in Aging (AG-NS-0521–0).

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Authors and Affiliations

  1. The Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel

    Sivan Henis-Korenblit

  2. Department of Biology, Queens College, The City University of New York, Flushing, NY, USA

    Alicia Meléndez

  3. Biology and Biochemistry PhD Programs, The Graduate Center of the City University of New York, New York, NY, USA

    Alicia Meléndez

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  1. Sivan Henis-Korenblit
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Correspondence to Sivan Henis-Korenblit or Alicia Meléndez .

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  1. Signalling Programme, Babraham Institute, Cambridge, UK

    Nicholas Ktistakis

  2. Signalling Programme, Babraham Institute, Cambridge, UK

    Oliver Florey

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Henis-Korenblit, S., Meléndez, A. (2019). Methods to Determine the Role of Autophagy Proteins in C. elegans Aging. In: Ktistakis, N., Florey, O. (eds) Autophagy. Methods in Molecular Biology, vol 1880. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8873-0_37

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  • DOI: https://doi.org/10.1007/978-1-4939-8873-0_37

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8872-3

  • Online ISBN: 978-1-4939-8873-0

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