Abstract
Liver macrophages (LMs) are phagocytic cells that play an important role in many liver disorders due to their ability to respond to a variety of stimuli and activating signals.
It is currently debated whether LMs activation from an anti-inflammatory to a proinflammatory phenotype contributes to obesity-induced metabolic diseases. We recently found that LMs can produce noninflammatory factors, such as the protein IGFBP7, able to directly regulate hepatic glucose production and lipid accumulation in the liver. However, while in a mouse model of obesity and insulin resistance LM-Igfbp7 expression is pathologically increased, in obese insulin-resistant patients LM-IGFBP7 is edited at RNA level independently of an effect on its expression. This discrepancy between results in animals and humans confirms the importance to perform molecular investigation directly on human’s isolated cells. Here, we describe a protocol to isolate liver macrophages from human liver biopsy .
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bellentani S (2017) The epidemiology of non-alcoholic fatty liver disease. Liver Int 37(Suppl 1):81–84. https://doi.org/10.1111/liv.13299
Morgantini C, Jager J, Li X et al (2019) Liver macrophages regulate metabolism through non-inflammatory factors. Nat Metab 1:445–459
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Morgantini, C. (2020). Kupffer Cell Isolation from Human Biopsies. In: Aouadi, M., Azzimato, V. (eds) Kupffer Cells. Methods in Molecular Biology, vol 2164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0704-6_2
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0704-6_2
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0703-9
Online ISBN: 978-1-0716-0704-6
eBook Packages: Springer Protocols